1921
Volume 74, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

To obtain the genetic structure of populations in the northern and southern malaria-endemic areas in Iran, which differ in endemicity, sequence diversity in the variable block 5 and the C-terminal part of merozoite surface protein 1 (Pvmsp 1) was analyzed. The variable block 5 fragment from 52 northern and 94 southern isolates was amplified and sequenced. Type 1, type 2, and recombinant type 3 allelic variants were found in both northern and southern isolates, with type 1 predominant in parasites from the north and type 2 in those from the south. A total of 7 and 27 distinct variants were detected among northern and southern isolates, respectively. A single variant predominated (71%) in the northern isolates, whereas variants were evenly distributed among southern isolates, with only two exceeding 10%. Thus, parasites from the southern malaria-endemic area were more polymorphic than those circulating in the northern area, where malaria is a re-emerging disease. Sequence alignments showed that although some variants were found only in northern or southern isolates, some were common to both and had also been observed in parasites from Azerbaijan, Turkey, Thailand, Bangladesh, and China. The Pvmsp 1 fragment corresponding to the C-terminal region was also amplified and the sequences derived from 20 northern and 50 southern isolates were identical. This high degree of conservation reinforces the potential of this polypeptide fragment for inclusion in synthetic vaccines being developed against

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2006-03-01
2017-11-21
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References

  1. Mendis K, Sina BJ, Marchesini P, Carter R, 2001. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 64 (Suppl): 97–106.
  2. Levitus G, Del Portillo HA, 1994. Advance toward the development of an asexual blood stage MSP-1 vaccine of Plasmodium vivax. Mem Inst Oswaldo Cruz 89 (Suppl): 81–84.
  3. Barat LM, Bloland PB, 1997. Drug resistance among malaria and other parasites. Infect Dis Clin North Am 11 : 969–987.
  4. Nomura T, Carlton JM, Baird JK, Del Portillo HA, Fryauff DJ, Rathore D, Fidock DA, Su X, Collins WE, McCutchan TF, Wootton JC, Wellems TE, 2001. Evidence for different mechanisms of chloroquine resistance in two Plasmodium species that cause human malaria. J Infect Dis 183 : 1653–1661.
  5. Cui L, Escalante AA, Imwongm M, Snounou G, 2003. The genetic diversity of Plasmodium vivax populations. Trends Parasitol 19 : 220–226.
  6. Zakeri S, Talebi Najafabadi S, Zare M, Djadid ND, 2002. Detection of malaria parasites by nested-PCR in southeastern Iran: evidence of highly mixed infections in Chabahar district. Malar J 1 : 2–6.
  7. Zakeri S, Mamaghani S, Mehrizi AA, Shahsavari Z, Raeisi A, Arshi S, Dinparast-Djadid N, 2004. Molecular evidence of mixed Plasmodium vivax and P. falciparum infections in northern Islamic Republic of Iran. East Mediterr Health J 10 : 336–342.
  8. del Portillo HA, Longacre S, Khouri E, David PH, 1991. Primary structure of the merozoite surface antigen 1 of Plasmodium vivax reveals sequences conserved between different Plasmodium species. Proc Natl Acad Sci USA 88 : 4030–4034.
  9. Gibson HL, Tucker JE, Kaslow DC, Kreltli AU, Collins WE, Kiefer MC, Bathurst IC, Barr PJ, 1992. Structure and expression of gene for PV200, a major blood-stage surface antigen of Plasmodium vivax. Mol Biochem Parasitol 50 : 325–334.
  10. Premawansa S, Snewin VA, Khouri E, Mendis KN, David PH, 1993. Plasmodium vivax: recombination between potential allelic types of the merozoite surface protein MSP1 in parasites isolated from patients. Exp Parasitol 76 : 192–199.
  11. Lim CS, Kim SH, Kwon SI, Song JK, Lee KN, 2000. Analysis of Plasmodium vivax merozoite surface protein-1 gene sequence from resurgent Korean isolates. Am J Trop Med Hyg 62 : 261–265.
  12. Gutierrez A, Vicini J, Patarroyo ME, Murillo LA, Patarroyo MA, 2000. Plasmodium vivax: polymorphism in the merozoite surface protein 1 gene from wild Colombian isolates. Exp Parasitol 95 : 215–219.
  13. Cheng Q, Stowers A, Huang TY, Bustos D, Huang YM, Rzepczyk C, Saul A, 1993. Polymorphism in Plasmodium vivax MSA1 gene. The result of intragenic recombinations? Parasitology 106 : 335–345.
  14. Craig AA, Kain KC, 1999. Molecular analysis of strains of Plasmodium vivax from paired primary and relapse infections. J Infect Dis 174: 373–379.
  15. Mancilla LI, Levituse G, Kirchgatter K, Mertens F, Herrera S, Del Portillo HA, 1994. Plasmodium vivax: dimorphic DNA sequences from the MSP-1 gene code for regions that are immunogenic in natural infections. Exp Parasitol 79 : 148–158.
  16. Kolakovich KA, Ssengoba A, Wojeik K, Tsuboi T, Alyaman F, Alpers M, Adamas JH, 1996. Plasmodium vivax: favored gene frequencies of the merozoite surface protein-1 and the multiplicity of infection in a malaria endemic region. Exp Parasitol 83 : 11–18.
  17. Imwong M, Pukrittayakamee S, Gruner AC, Renia L, Letourneur F, Looareesuwan S, White NJ, Snounou G, 2005. Practical PCR genotyping protocols for Plasmodium vivax using Pvcs and Pvmsp1. Malar J 4 : 20.
  18. O’Donnell RA, Saul A, Cowman AF, Crabb BS, 2000. Functional conservation of the malaria vaccine antigen MSP119 across distantly related Plasmodium species. Nat Med 6 : 91–95.
  19. Han HJ, Park SG, Kim SH, Hwang SY, Han J, Traicoff J, Kho WG, Chung JY, 2004. Epidermal growth factor-like motifs 1 and 2 of Plasmodium vivax merozoite surface protein 1 are critical domains in erythrocyte invasion. Biochem Biophys Res Commun 320 : 563–570.
  20. Snounou G, Viriyakosol S, Jarra W, Pinherio L, Do Rsario VE, Thithong S, Brown KN, 1993. High sensitivity of detection of human malaria parasites by use of nested polymerase chain reaction. Mol Biochem Parasitol 61 : 315–320.
  21. Putaporntip C, Jongwutiwes S, Sakihama S, Ferreira MU, Kho W-G, Kaneko A, Kanbara H, Hattori T, Tanabe K, 2002. Mosaic organization and heterogeneity in frequency of allelic recombination of the Plasmodium vivax merozoite surface protein-1 locus. Proc Natl Acad Sci USA 99 : 16348–16353.
  22. Kumar S, Tamura K, Nei M, 1993. MEGA: Molecular Evolutionary Genetics Analysis. University Park, PA: Pennsylvania State University.
  23. Pasay MC, Cheng Q, Rzepczyk C, Saul A, 1995. Dimorphism of the C terminus of the Plasmodium vivax merozoite surface protein 1. Mol Biochem Parasitol 70 : 217–219.
  24. Leclerc MC, Menegon M, Cligny A, Noyer JL, Mammadov S, Aliyev N, Gasimov E, Majori G, Severini C, 2004. Genetic diversity of Plasmodium vivax isolates from Azerbaijan. Malaria J 3 : 40.
  25. Maestre A, Sunil S, Ahmad G, Mohmmed A, Echeverri M, Corredor M, Blair S, Chauhan VS, Malhotra P, 2004. Inter-allelic recombination in the Plasmodium vivax merozoite surface protein 1 gene among Indian and Colombian isolates. Malar J 3: 4.
  26. Arez AP, Snounou G, Pinto J, Sousa CA, Modiano D, Ribeiro H, Franco AS, Alves J, Do Rosario VE, 1999. A clonal Plasmodium falciparum population in an isolated outbreak of malaria in the republic of Cabo Verde. Parasitology 118 : 347–355.
  27. Mann VH, Good MF, Saul A, 1995. Diversity in the circumsporozoite protein of Plasmodium vivax: does it matter? Parasitol Today 11 : 33–36.
  28. Qari SH, Goldman LF, Povoa MM, Oliverira S, Alpers MP, Lal AA, 1991. Wide distribution of the variant form of human malaria parasite Plasmodium vivax. J Biol Chem 266 : 16297–16300.
  29. Bruce MC, Galinski MR, Barnwell JW, Snounou G, Day KP, 1999. Polymorphism at the merozoite protein 3α locus of Plasmodium vivax: global and local diversity. Am J Trop Med Hyg 61 : 518–525.
  30. Gomez JC, McNamara DT, Bockarie MJ, Baird JK, Carlton JM, Zimmerman PA, 2003. Identification of a polymorphic Plasmodium vivax microsatellite marker. Am J Trop Med Hyg 69 : 377–379.
  31. Feng X, Carlton JM, Joiy DA, Mu J, Furuya T, Suh BB, Wang Y, Barnwell JW, Su XZ, 2003. Single-nucleotide polymorphisms and genome diversity in Plasmodium vivax. Proc Natl Acad Sci USA 100 : 8502–8507.
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  • Received : 12 May 2005
  • Accepted : 31 Oct 2005

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