1921
Volume 74, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Malaria vaccines based on thrombospondin-related adhesive protein of ( TRAP) are currently undergoing clinical trials in humans. This study was designed to investigate naturally acquired cellular immunity to TRAP in adults from a target population for future trials of TRAP-based vaccines in Kilifi, Kenya. We first tested reactivity to a panel of 53 peptides spanning TRAP and identified 26 novel T-cell epitopes. A panel of naturally occurring polymorphic variant epitope peptides were made to the most commonly recognized epitope regions and tested for ability to elicit IFN-γ, IL-4, and IL-10 production. These data provide for the first time a complex cytokine matrix mapping naturally induced T-cell responses to TRAP and suggest that T-cell responses boosted by vaccination with TRAP could stimulate the release of competing pro- and anti-inflammatory cytokines. They further define polymorphic variants able to boost specific Th1, Th2, and possibly Tr1 reactivity.

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2006-03-01
2017-11-25
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  • Received : 09 Feb 2005
  • Accepted : 09 Jul 2005

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