Volume 74, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Visceral leishmaniasis (VL) is caused by various strains of , and with different geographical distribution. The aim of this study was to identify the strains of that can cause VL in southern Iran. DNA of were extracted from the slides of bone marrow aspirates (#42) and spleen punctures (#22), which were positive for leishman body from the patients who were referred to the hospitals affiliated with Shiraz University of Medical Sciences. Differences in strains were determined by size difference of the polymerase chain reaction (PCR) amplification as visualized on agarose gel. PCR results and smears had 100% correlation. The dominant strain of was (63 out of the 64 cases), but one case of was also detected. VL mostly involves children below 2 years of age in Iran, therefore infection with was expected, but this study is the first report of VL that is caused by in Iran.


Article metrics loading...

Loading full text...

Full text loading...



  1. Hashemi-Nasab A, Zadeh-Shirazi H, 1980. Visceral leishmaniasis in Fars province; IRAN: study of 130 cases. J Trop Med Hyg 83 : 119–122.
  2. Edrisian GH, Nadim A, Alborzi A, Ardehali S, 1998. Visceral leishmaniasis; the Iranian experience. Arch Irn Med 1 : 22–26.
  3. Nadim A, Navid-Hamidid A, Javadian E, Bidruni GT, Amini H, 1978. Present status of Kalaazar in Iran. Am J Trop Med Hyg 27 (1 pt 1): 25–28.
  4. Magill AJ, Grogl M, Johnson SC, Gasser RA Jr, 1994. Visceral infection due to Leishmania tropica in a veteran of operation Desert Storm who presented 2 years after leaving Saudi Arabia. Clin Infect Dis 19 : 805–806.
  5. Boyer MH, 1993. Visceral leishmaniasis in Desert Storm veterans. N Engl J Med 329 : 1503–1504.
  6. Magill AJ, Grogl M, Gasser RA Jr, Sun W, Oster CN, 1993. Visceral infection caused by Leishmania tropica in veterans of operation Desert Storm. N Engl J Med 328 : 1383–1387.
  7. Noyes HA, Reyburn H, Bailey JW, Smith D, 1998. A nested-PCR Schizodem method for identifying Leishmania kinetoplast minicircle classes directly from clinical samples and its application to the study of the epidemiology of L. tropica in Pakistan. J Clin Microbiol 36 : 2877–2881.
  8. Mebrahtu Y, Lawyer P, Githure J, Were JB, Muigai R, Hendricks L, Leeuwenburg J, Koech D, Roberts C, 1989. Visceral Leishmaniasis unresponsive to pentostam caused by Leishmania tropica in Kenya. Am J Trop Med Hyg 41 : 289–294.
  9. Sackes DL, Kenney RT, Kreutzer RD, Jaffe CL, Gupta AK, Sharma MC, Sinha SP, Neva FA, Saran R, 1995. Indian Kalaazar caused by Leishmania tropica. Lancet 345 : 959–961.
  10. Guessous-Idrissi N, Berrag B, Riyad M, Sahibi H, Bichichi M, Rhalem A, 1997. Leishmania tropica: etiologic agent of a case of canine visceral leishmaniasis in northern Morocco. Am J Trop Med Hyg 57 : 172–173.

Data & Media loading...

  • Received : 08 Sep 2004
  • Accepted : 22 Mar 2005

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error