1921
Volume 74, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Sulfadoxine-pyrimethamine (SP) is the second-line treatment for malaria in Sri Lanka. Resistance to SP is caused by point mutations in the dihydrofolate reductase () and dihydropteroate synthase (-) genes of . We determined the genotype of and and the clinical response to SP in 30 field isolates of from Sri Lanka. All patients treated with SP had an adequate clinical response. Eighty-five percent (23 of 27) of pure field isolates carried parasites with double mutant alleles of (C59R + S108N) and showed about 200-fold higher levels of resistance to pyrimethamine than the wild type in a yeast system. None of the isolates had either known or novel mutations at other positions in the domain. In contrast, 67% (20 of 30) of the isolates carried parasites that were wild type for . In Sri Lanka, detection of the triple mutant allele of will require tracking mutations at codon 51.

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2006-02-01
2017-11-18
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  • Received : 24 Mar 2005
  • Accepted : 06 Oct 2005

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