1921
Volume 74, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

serine repeat antigen (SERA5) is a promising asexual blood stage malaria candidate vaccine. However, there is a paucity of information about natural immune responses to SERA5 in children from malaria-endemic regions. We undertook a hospital-based case-control study of severe malaria in Apac District, Northern Uganda, in children 6–59 months of age. The commonest symptoms observed in children with severe malaria (SM) were respiratory distress (53.4%) and prostration (40.4%) followed by circulatory collapse (7.4%), severe anemia (Hb < 5 g/dL, 7.0%), and seizures (2.6%). None of the SM children had impaired consciousness, coma, or cerebral malaria. We measured serum IgG antibodies using a recombinant construct of SERA5 (SE36) in enzyme-linked immunosorbent assays. High titers of IgG anti-SE36 were associated with protection against severe malaria in children under 5 years old.

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References

  1. Guyatt HL, Snow RW, 2001. Malaria in pregnancy as an indirect cause of infant mortality in sub-Saharan Africa. Trans R Soc Trop Med Hyg 95 : 569–576. [Google Scholar]
  2. Kasumba IN, Nalunkuma AJ, Mujuzi G, Kitaka FS, Byaruhanga R, Okong P, Egwang TG, 2000. Low birthweight associated with maternal anaemia and Plasmodium falciparum infection during pregnancy, in a peri-urban/urban area of low endemicity in Uganda. Ann Trop Med Parasitol 94 : 7–13. [Google Scholar]
  3. Maitland K, Makanga M, Williams TN, 2004. Falciparum malaria: current therapeutic challenges. Curr Opin Infect Dis 17 : 405–412. [Google Scholar]
  4. Chandre F, Darrier F, Manga L, Akogbeto M, Faye O, Mouchet J, Guillet P, 1999. Status of pyrethroid resistance in Anopheles gambiae sensu lato. Bull World Health Organ 77 : 230–234. [Google Scholar]
  5. Aoki S, Li J, Itagaki S, Okech BA, Egwang TG, Matsuoka H, Palacpac NM, Mitamura T, Horii T, 2002. Serine repeat antigen (SERA5) is predominantly expressed among the SERA multigene family of Plasmodium falciparum, and the acquired antibody titers correlate with serum inhibition of the parasite growth. J Biol Chem 277 : 47533–47540. [Google Scholar]
  6. Inselburg J, Bathurst IC, Kansopon J, Barchfeld GL, Barr PJ, Rossan RN, 1993. Protective immunity induced in Aotus monkeys by a recombinant SERA protein of Plasmodium falciparum: adjuvant effects on induction of protective immunity. Infect Immun 61 : 2041–2047. [Google Scholar]
  7. Inselburg J, Bzik DJ, Li WB, Green KM, Kansopon J, Hahm BK, Bathurst IC, Barr PJ, Rossan RN, 1991. Protective immunity induced in Aotus monkeys by recombinant SERA proteins of Plasmodium falciparum. Infect Immun 59 : 1247–1250. [Google Scholar]
  8. Enders B, Hundt E, Knapp B, 1992. Protection of Aotus monkeys after immunization with recombinant antigens of Plasmodium falciparum. Mem Inst Oswaldo Cruz 87 (Suppl 3): 413–422. [Google Scholar]
  9. Pang XL, Horii T, 1998. Complement-mediated killing of Plasmodium falciparum erythrocytic schizont with antibodies to the recombinant serine repeat antigen (SERA). Vaccine 16 : 1299–1305. [Google Scholar]
  10. Pang XL, Mitamura T, Horii T, 1999. Antibodies reactive with the N-terminal domain of Plasmodium falciparum serine repeat antigen inhibit cell proliferation by agglutinating merozoites and schizonts. Infect Immun 67 : 1821–1827. [Google Scholar]
  11. Sugiyama T, Suzue K, Okamoto M, Inselburg J, Tai K, Horii T, 1996. Production of recombinant SERA proteins of Plasmodium falciparum in Escherichia coli by using synthetic genes. Vaccine 14 : 1069–1076. [Google Scholar]
  12. Okech BA, Nalunkuma A, Okello D, Pang XL, Suzue K, Li J, Horii T, Egwang TG, 2001. Natural human immunoglobulin G subclass responses to Plasmodium falciparum serine repeat antigen in Uganda. Am J Trop Med Hyg 65 : 912–917. [Google Scholar]
  13. Egwang TG, Apio B, Riley E, Okello D, 2000. Plasmodium falciparum malariometric indices in Apac district, northern Uganda. East Afr Med J 77 : 413–416. [Google Scholar]
  14. Apio B, Nalunkuma A, Okello D, Riley E, Egwang TG, 2000. Human IgG subclass antibodies to the 19 kilodalton carboxy terminal fragment of Plasmodium falciparum merozoite surface protein 1 (MSP1(19)) and predominance of the MAD20 allelic type of MSP1 in Uganda. East Afr Med J 77 : 189–193. [Google Scholar]
  15. Premji Z, Hamisi Y, Shiff C, Minjas J, Lubega P, Makwaya C, 1995. Anaemia and Plasmodium falciparum infections among young children in an holoendemic area, Bagamoyo, Tanzania. Acta Trop 59 : 55–64. [Google Scholar]
  16. Biemba G, Dolmans D, Thuma PE, Weiss G, Gordeuk VR, 2000. Severe anaemia in Zambian children with Plasmodium falciparum malaria. Trop Med Int Health 5 : 9–16. [Google Scholar]
  17. World Health Organization Communicable Diseases Cluster, 2000. Severe falciparum malaria. Trans R Soc Trop Med Hyg 94 (Suppl): S1–90. [Google Scholar]
  18. Snow RW, Nahlen B, Palmer A, Donnelly CA, Gupta S, Marsh K, 1998. Risk of severe malaria among African infants: direct evidence of clinical protection during early infancy. J Infect Dis 177 : 819–822. [Google Scholar]
  19. Snow RW, Omumbo JA, Lowe B, Molyneux CS, Obiero JO, Palmer A, Weber MW, Pinder M, Nahlen B, Obonyo C, New-bold C, Gupta S, Marsh K, 1997. Relation between severe malaria morbidity in children and level of Plasmodium falciparum transmission in Africa. Lancet 349 : 1650–1654. [Google Scholar]
  20. Jensen JB, Boland MT, Akood M, 1982. Induction of crisis forms in cultured Plasmodium falciparum with human immune serum from Sudan. Science 216 : 1230–1233. [Google Scholar]
  21. Ayi K, Turrini F, Piga A, Arese P, 2004. Enhanced phagocytosis of ring-parasitized mutant erythrocytes: a common mechanism that may explain protection against falciparum malaria in sickle trait and beta-thalassemia trait. Blood 104 : 3364–3371. [Google Scholar]
  22. Whitworth J, Morgan D, Quigley M, Smith A, Mayanja B, Eotu H, Omoding N, Okongo M, Malamba S, Ojwiya A, 2000. Effect of HIV-1 and increasing immunosuppression on malaria parasitaemia and clinical episodes in adults in rural Uganda: a cohort study. Lancet 356 : 1051–1056. [Google Scholar]
  23. Erunkulu OA, Hill AV, Kwiatkowski DP, Todd JE, Iqbal J, Berzins K, Riley EM, Greenwood BM, 1992. Severe malaria in Gambian children is not due to lack of previous exposure to malaria. Clin Exp Immunol 89 : 296–300. [Google Scholar]
  24. Hodder AN, Drew DR, Epa VC, Delorenzi M, Bourgon R, Miller SK, Moritz RL, Frecklington DF, Simpson RJ, Speed TP, Pike RN, Crabb BS, 2003. Enzymic, phylogenetic, and structural characterization of the unusual papain-like protease domain of Plasmodium falciparum SERA5. J Biol Chem 278 : 48169–48177. [Google Scholar]
  25. Lyke KE, Diallo DA, Dicko A, Kone A, Coulibaly D, Guindo A, Cissoko Y, Sangare L, Coulibaly S, Dakouo B, Taylor TE, Doumbo OK, Plowe CV, 2003. Association of intraleukocytic Plasmodium falciparum malaria pigment with disease severity, clinical manifestations, and prognosis in severe malaria. Am J Trop Med Hyg 69 : 253–259. [Google Scholar]
  26. Amodu OK, Adeyemo AA, Olumese PE, Gbadegesin RA, 1998. Intraleucocytic malaria pigment and clinical severity of malaria in children. Trans R Soc Trop Med Hyg 92 : 54–56. [Google Scholar]
  27. Luty AJ, Perkins DJ, Lell B, Schmidt-Ott R, Lehman LG, Luckner D, Greve B, Matousek P, Herbich K, Schmid D, Weinberg JB, Kremsner PG, 2000. Low interleukin-12 activity in severe Plasmodium falciparum malaria. Infect Immun 68 : 3909–3915. [Google Scholar]
  28. Silamut K, White NJ, 1993. Relation of the stage of parasite development in the peripheral blood to prognosis in severe falciparum malaria. Trans R Soc Trop Med Hyg 87 : 436–443. [Google Scholar]
  29. Silamut K, Phu NH, Whitty C, Turner GD, Louwrier K, Mai NT, Simpson JA, Hien TT, White NJ, 1999. A quantitative analysis of the microvascular sequestration of malaria parasites in the human brain. Am J Pathol 155 : 395–410. [Google Scholar]
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  • Received : 10 Feb 2005
  • Accepted : 14 Apr 2005

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