Volume 74, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


The clinical laboratory, virologic, and pathologic changes occurring in hamsters after infection with Pirital virus () are described. Pirital virus infection in the hamsters was characterized by high titered viremia, leukocytosis, coagulopathy, pulmonary hemorrhage and edema, hepatocellular and splenic necrosis, and marked elevation of serum transaminase levels. All of the animals died within 9 days. The clinical and histopathological findings in the Pirital virus–infected hamsters were very similar to those reported in severe human cases of Lassa fever, suggesting that this new animal model could serve as a low-cost and relatively safe alternative for studying the pathogenesis and therapy of Lassa fever.


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  1. Peters CJ, 2002. Arenaviruses. Richman DD, Whitley RJ, Hayden FG, eds. Clinical Virology. Second edition. Washington, DC: ASM Press, 949–969.
  2. McCormick JB, Fisher-Hoch SP, 2002. Lassa fever. Curr Top Microbiol Immunol 262 : 75–109. [Google Scholar]
  3. Webb PA, Maiztegui J, 1988. Argentine and Bolivian hemorrhagic fevers (South American hemorrhagic fevers). Gear JHS, ed. CRC Handbook of Viral and Rickettsial Hemorrhagic Fevers. Boca Raton, FL: CRC Press, 145–154.
  4. de Manzione N, Salas RA, Paredes H, Godoy O, Rojas L, Araoz F, Fulhorst CF, Ksiazek TG, Mills JN, Ellis BA, Peters CJ, Tesh RB, 1998. Venezuelan hemorrhagic fever: clinical and epidemiological studies of 165 cases. J Infect Dis 26 : 308–313. [Google Scholar]
  5. Coimbra TLM, Nassar ES, Burattini MN, de Souza LTM, Ferreira IB, Rocco IM, Travassos da Rosa APA, Vasconcelos PFC, Pinheiro FP, Le Duc JW, Rico-Hesse R, Gonzalez JP, Jahrling PB, Tesh RB, 1994. New arenavirus isolated in Brazil. Lancet 343 : 391–392. [Google Scholar]
  6. Peters CJ, Jahrling PB, Kiu CT, Kenyon RH, McKee KT, Barrera Oro JB, 1987. Experimental studies of arenaviral hemorrhagic fevers. Curr Top Microbiol Immunol 134 : 5–68. [Google Scholar]
  7. Jahrling PB, Hesse RA, Eddy GA, Johnson KM, Callis RT, Stephen EL, 1980. Lassa virus infection of rhesus monkeys: pathogenesis and treatment with ribavirin. J Infect Dis 141 : 580–588. [Google Scholar]
  8. Jahrling PB, Smith S, Hesse RA, Rhoderick JB, 1982. Pathogenesis of Lassa virus infection in guinea pigs. Infect Immun 37 : 371–378. [Google Scholar]
  9. Hall WC, Geisbert TW, Huggins JW, Jahrling PB, 1996. Experimental infection of guinea pigs with Venezuelan hemorrhagic fever virus (Guanarito): a model of human disease. Am J Trop Med Hyg 55 : 81–88. [Google Scholar]
  10. Connolly BM, Jenson AB, Peters CJ, Geyer SJ, Barth JF, McPherson RA, 1993. Pathogenesis of Pichinde virus infection in strain 13 guinea pigs: an immunologic, virologic and clinical chemistry study. Am J Trop Med Hyg 49 : 10–24. [Google Scholar]
  11. Kastello MD, Eddy GA, Kuehne RW, 1976. A rhesus monkey model for the study of Bolivian hemorrhagic fever. J Infect Dis 133 : 57–62. [Google Scholar]
  12. Walker DH, Wulff H, Murphy FA, 1975. Experimental Lassa virus infection in the squirrel monkey. Am J Pathol 80 : 261–272. [Google Scholar]
  13. Aronson JF, Herzog NK, Jerrells TR, 1994. Pathological and virological features of arenavirus disease in guinea pigs. Comparison of two Pichinde virus strains. Am J Pathol 145 : 228–235. [Google Scholar]
  14. Cosgriff TM, Jahrling PB, Chen J, Hodgson LA, Lewis RM, Green DE, Smith JI, 1987. Studies of the coagulation system in arenaviral hemorrhagic fever: experimental infection of strain 13 guinea pigs with Pichinde virus. Am J Trop Med Hyg 36 : 416–423. [Google Scholar]
  15. Fisher-Hoch SP, Mitchell SW, Sasso DR, Lange JV, Ramsey R, McCormick JB, 1987. Physiologic and immunologic disturbances associated with shock in a primate model of Lassa fever. J Infect Dis 155 : 465–474. [Google Scholar]
  16. Lange JV, Mitchell SW, McCormick JB, Walker DH, Evatt BL, Ramsey RR, 1985. Kinetic study of platelets and fibrinogen in Lassa virus-infected monkeys and early pathologic events in Mopeia virus-infected monkeys. Am J Trop Med Hyg 34 : 999–1007. [Google Scholar]
  17. Xiao SY, Zhang H, Yang Y, Tesh RB, 2001. Pirital virus (Arenaviridae) infection in the Syrian golden hamster, Mesocricetus auratus: a new animal model for arenaviral hemorrhagic fever. Am J Trop Med Hyg 64 : 111–118. [Google Scholar]
  18. Fulhorst CF, Bowen MD, Salas RA, de Manzione NMC, Duno G, Utrera A, Ksiazek TG, Peters CJ, Nichol ST, de Miller E, Tovar D, Ramos B, Vasquez C, Tesh RB, 1997. Isolation and characterization of Pirital virus, a newly discovered South American arenavirus. Am J Trop Med Hyg 56 : 548–553. [Google Scholar]
  19. Salvato MS, Clegg JCS, Buchmeier MJ, Charrel RN, Gonzalez J-P, Lukashevich IS, Peters CJ, Rico-Hesse R, Ramanowski V, 2005. Family Arenaviridae. Fauguet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA, eds. Virus Taxonomy: Classification and Nomenclature of Viruses. Eight Report of the International Committee on the Taxonomy of Viruses. San Diego, CA: Elsevier Academic Press, 725–733.
  20. Xiao SY, Zhang H, Guzman H, Tesh RB, 2001. Experimental yellow fever virus infection in the golden hamster (Mesocricetus auratus). II. Pathology. J Infect Dis 183 : 1437–1444. [Google Scholar]
  21. CDC/NIH, 1999. Biosafety in Microbiological and Biomedical Laboratories. Fourth edition. Washington, DC: U.S. Government Printing Office.
  22. Monath TP, Maher M, Casals J, Kissling RE, Cacciapuoti A, 1974. Lassa fever in the eastern province of Sierra Leone, 1970–1972. II. Clinical observations and virological studies on selected hospital cases. Am J Trop Med Hyg 23 : 1140–1149. [Google Scholar]
  23. McCormick JB, Walker DH, King IJ, Webb PA, Elliott LH, Whitfield SG, Johnson KM, 1986. Lassa virus hepatitis: A study of fatal Lassa fever in humans. Am J Trop Med Hyg 35 : 401–407. [Google Scholar]
  24. McCormick JB, King IJ, Webb PA, Johnson KM, O’Sullivan R, Smith ES, Trippel S, Tong TC, 1987. A case-control study of the clinical diagnosis and course of Lassa fever. J Infect Dis 155 : 445–454. [Google Scholar]
  25. Walker DH, McCormick JB, Johnson KM, Webb PA, Komba-Kono G, Elliot LH, Garner JJ, 1982. Pathologic and virologic study of fatal Lassa fever in man. Am J Path 107 : 349–356. [Google Scholar]
  26. Walker DH, Wulff H, Lange JV, Murphy FA, 1975. Comparative pathology of Lassa virus infection in monkeys, guinea-pigs and Mastomys natalensis. Bull WHO 52 : 523–534. [Google Scholar]
  27. Fisher-Hoch SP, 1993. Arenavirus pathophysiology. Salvato MS, ed. The Arenaviridae. New York: Plenum Press, 299–323.

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  • Received : 18 Jan 2006
  • Accepted : 17 Feb 2006

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