Volume 74, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


We assessed the seroprevalence of infection using indirect hemagglutination antibody (IHA) assay among 667 HIV-infected persons (group A), 1,311 asymptomatic HIV-uninfected persons seeking anonymous HIV testing (group B), 616 HIV-uninfected controls with gastrointestinal symptoms (diarrhea and/or liver abscess) seeking medical care (group C), and 2,500 healthy controls undergoing health check-up (group D). An IHA titer ≥ 128 was detected in 7.1% of group A, 2.5% of group B, 1.8% of group C, and 0.1% of group D ( < 0.0001). The highest seroprevalence (11.2%) was noted among HIV-infected persons who were men having sex with men (MSM) 30–39 years of age. Compared with persons with gastrointestinal symptoms, the adjusted odds ratio for having high IHA titers among HIV-infected persons was 3.206 (95% confidence interval, 1.433, 7.176; = 0.005). These findings show that HIV-infected persons, especially MSM 30–39 years of age, are at significantly higher risk of infection.


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  1. Keystone JS, Keystone DL, Proctor EM, 1980. Intestinal parasitic infections in homosexual men: prevalence, symptoms and factors in transmission. CMAJ 123 : 512–514.
  2. Phillips SC, Mildvan D, William DC, Gelb AM, White AC, 1981. Sexual transmission of enteric protozoa and helminths in a venereal-disease-clinic population. N Engl J Med 305 : 603–606.
  3. Quinn TC, Stamm WE, Goodell SE, Mkrtichian E, Benedetti J, Corey L, Schuffler MD, Holmes KK, 1983. The polymicrobial origin of intestinal infections in homosexual men. N Engl J Med 309 : 576–582.
  4. Markell EK, Hanens RF, Kuritsubo RA, Wingerd J, 1984. Intestinal protozoa in homosexual men of the San Francisco Bay area: prevalence and correlates of infection. Am J Trop Med Hyg 33 : 239–245.
  5. Allason-Jones E, Mindel A, Sargenunt P, Williams P, 1986. Entamoeba histolytica as a commensal intestinal parasite in homosexual men. N Engl J Med 315 : 353–356.
  6. Lowther SA, Dworkin MS, Hanson DL, and the Adult and Adolescent Spectrum of Human Immunodeficiency Virus Disease Project, 2000. Entamoeba histolytica/Entamoeba dispar in human immunodeficiency virus-infected patients in the United States. Clin Infect Dis 30 : 955–959.
  7. Aceti A, Pennica A, Ippolito G, Moretto D, Rezza G, Titti F, Perucci CA, 1987. Antiamebic antibodies in homosexual men. N Engl J Med 316 : 692.
  8. Takeuchi T, Okuzawa E, Nozaki T, Kobayashi S, Mizokami M, Minoshima N, Yamamoto M, Isomura S, 1989. High seropositivity of Japanese homosexual men for amebic infection. J Infect Dis 159 : 808.
  9. Takeuchi T, Miyahira Y, Kobyash S, Nozaki T, Motta S, Matsuda L, 1990. High seropositivity for Entamoeba histolytica infection in Japanese homosexual men: further evidence for occurrence of pathogenic strains. Am J Trop Med Hyg 84 : 250–251.
  10. Hung CC, Chen PJ, Hsieh SM, Wong JM, Chen MY, Chang SC, 1999. Invasive amebiasis: an emerging parasitic disease in patients with HIV infection in an endemic area of amebic infection. AIDS 13 : 2421–2428.
  11. Mitarai S, Nagai H, Satoh K, Hebisawa A, Shishido H, 2001. Amebiasis in Japanese homosexual men with human immunodeficiency virus infection. Intern Med 40 : 671–675.
  12. Ohnishi K, Kato Y, Imamura A, Fukayama M, Tsunoda T, Sakaue Y, Sakamoto M, Sagara H, 2004. Present characteristics of symptomatic Entamoeba histolytica infection in the big cities of Japan. Epidemiol Infect 132 : 57–60.
  13. Hung CC, Deng HI, Hsiao WH, Hsieh SM, Hsiao CF, Chen MY, Chang SC, Su KE, 2005. Invasive amoebiasis is an emerging parasitic infection in patients with HIV infection. Arch Intern Med 165 : 409–415.
  14. Centers for Disease Control and Prevention, 1992. 1993 Revised classification system for HIV infection and expanded surveillance case definition for AIDS among adolescents and adults. MMWR Morb Mortal Wkly Rep 41 : 1–19.
  15. Haque R, Ali IM, Sack RB, Farr BM, Ramakrishnan G, Petri WA Jr, 2001. Amebiasis and mucosal IgA antibody against the Entamoeba histolytica adherence lectin in Bangladesh children. J Infect Dis 183 : 1787–1793.
  16. Haque R, Mollah NU, Ali IKM, Alam K, Eubanks A, Lyerly D, Petri WA Jr, 2000. Diagnosis of amebic liver abscess and intestinal infection with the Techlab Entamoeba histolytica II antigen detection and antibody tests. J Clin Microbiol 38 : 3235–3239.
  17. Blessmann J, Ali IK, Nu PA, Dinh BT, Viet TQ, Van AL, Clark CG, Tannich E, 2003. Longitudinal study of intestinal Entamoeba histolytica infections in asymptomatic adult carriers. J Clin Microbiol 41 : 4745–4750.
  18. Sorvillo FJ, Strassburg MA, Seidel J, Visvesvara GS, Mori K, Todd A, Portigal L, Finn M, Agee BA, 1986. Amebic infections in asymptomatic homosexual men: lack of evidence of invasive disease. Am J Public Health 76 : 1137–1139.
  19. Lee HC, Ko NY, Chang MP, Lee WC, Ko WC, 2005. Prevalence of HIV infection and other sexually transmitted diseases in gay saunas in Taiwan. Clin Microbiol Infect 11 (Suppl 2): 198.
  20. Moran P, Ramos F, Ramiro M, Curiel O, Gonzalez E, Valadez A, Gomez A, Garcia G, Melendro EI, Ximenez C, 2005. Infection by human immunodeficiency virus-1 is not a risk factor for amebiasis. Am J Trop Med Hyg 73 : 296–300.

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  • Received : 15 Nov 2005
  • Accepted : 19 Feb 2006

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