Volume 74, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


To identify clones from chronically infected individuals, they were transferred to triatomines by the xenodiagnosis test (XD) with . Polymerase chain reaction (PCR) and hybridization assays were performed to detect minicircle DNA in human blood samples and triatomine feces, using probes to determine the clones present. clone 19 TcI) resulted the most prevalent in humans, with a frequency of 0.70 compared with a frequency of 0.53 in triatomines. clone 39 (TcIId) was the most prevalent in , with a frequency of 0.65 compared with 0.33 in humans. The clone 43 (TcIIe) was not detected in blood samples; nevertheless, it was present at a rate of 0.17 in feces. In conclusion, the clones are associated to each host, suggesting that selective amplification of clones occurs in human and triatomines.


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  • Received : 29 Apr 2005
  • Accepted : 13 Jan 2006

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