Volume 73, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


The currently accepted gold standard for enumeration of leptospires is tabulation of organisms using a Petroff-Hausser counting chamber and dark-field microscopy, a technically demanding, time-consuming technique. Quantification of leptospires with a Coulter counter produced reliable and reproducible counts, comparable to the counting chamber. This experiment demonstrates that the faster, less technically demanding Coulter counter may be an alternative to determine numbers of leptospires.


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  • Received : 26 Apr 2005
  • Accepted : 27 May 2005
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