1921
Volume 73, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The currently accepted gold standard for enumeration of leptospires is tabulation of organisms using a Petroff-Hausser counting chamber and dark-field microscopy, a technically demanding, time-consuming technique. Quantification of leptospires with a Coulter counter produced reliable and reproducible counts, comparable to the counting chamber. This experiment demonstrates that the faster, less technically demanding Coulter counter may be an alternative to determine numbers of leptospires.

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/content/journals/10.4269/ajtmh.2005.73.962
2005-11-01
2017-11-19
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References

  1. Levett PN, 2001. Leptospirosis. Clin Microbiol Rev 14 : 296–326.
  2. Shieh WJ, Edwards C, Spiegel R, Zaki SR, 1999. Leptospirosis. Guerrant DL, Walker DH, Weller PF, eds. Tropical Infectious Diseases. Principles, Pathogens & Practice. Philadelphia: Churchill Livingstone, 547–555.
  3. Larson AD, Treick RW, Edwards CL, Cox CD, 1959. Growth studies and plate counting of leptospires. J Bacteriol 77 : 361–366.
  4. Faine S AB, Bolin C, Perolat P, 2000. Leptospira and Leptospirosis. Melbourne, Australia: MediSci, 173–174.
  5. Andrew MH, Westwood N, 1971. Use of the Coulter counter to count cells of Streptococcus faecalis. J Appl Bacteriol 34 : 441–447.
  6. Smither R, 1975. Use of a Coulter counter to detect discrete changes in cell numbers and volume during growth of Escherichia coli. J Appl Bacteriol 39 : 157–165.
  7. Li RC, Lee SW, Lam JS, 1996. Novel method for assessing post-antibiotic effect by using the Coulter counter. Antimicrob Agents Chemother 40 : 1751–1753.
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  • Received : 26 Apr 2005
  • Accepted : 27 May 2005

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