1921
Volume 73, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

A rapid and reliable diagnostic assay for acute hepatitis E virus (HEV) infection is needed. We evaluated a rapid, immunochromatographic assay for IgM antibodies to HEV (ASSURE™ HEV IgM Rapid Test) using acute-phase HEV samples (n = 200) from Indonesia and Nepal and convalescent-phase HEV samples (n = 70) from Nepal. Blood donors in Thailand (n = 100), individuals with hepatitis A (n = 80), hepatitis B (n = 45), and hepatitis C (n = 50) in Thailand and Nepal, acute-phase sera of individuals with Epstein-Barr virus infection (n = 20), and rheumatoid factor–positive blood (n = 26) served as negative controls. The assay had a sensitivity of 93% (95% confidence interval [CI] = 88.5–96.1%) and a specificity of 99.7% (95% CI = 98.3–100%). The positive and negative predictive values were 99.5% (95% CI = 97.1–100%) and 95.8% (95% CI = 93.1–97.7%), respectively. These results suggest that this assay is a sensitive and specific tool for the rapid diagnosis of acute HEV infection.

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2005-11-01
2017-11-24
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References

  1. Bradley DW, 1990. Enterically-transmitted non-A, non-B hepatitis. Br Med Bull 46 : 442–461.
  2. Bradley DW, 1990. Hepatitis non-A, non-B viruses become identified as hepatitis C and E viruses. Melnick JL, ed. Progress in Medical Virology. Volume 37. Basel: Karger, 101–135.
  3. Bradley DW, Krawczynski K, Cook EH Jr, McCaustland KA, Robertson BH, Humphrey CD, Kane MA, Spelbring J, Weisfuse I, Andjaparidze A, Balayan M, Stetler H, Velazquez O, 1998. Enterically transmitted non-A, non-B hepatitis: eitiology of disease and laboratory studies in nonhuman primates. Zuckerman AJ, ed. Viral Hepatitis and Liver Disease. New York: Alan R. Liss, Inc., 138–147.
  4. Purcell RH, Ticehurst JR, 1988. Enterically transmitted non-A, non-B hepatitis: epidemiology and clinical characteristics. Zuckerman AJ, ed. Viral Hepatitis and Liver Disease. New York: Alan R. Liss, Inc., 131–137.
  5. Ticehurst J, 1991. Identification and characterization of hepatitis E virus. Hollinger FB, Lemon SM, Margolis HS, eds. Viral Hepatitis and Liver Disease. Baltimore: Williams and Wilkins, 501–513.
  6. World Health Organization. Communicable Disease Surveillance and Response (CSR), Hepatitis E in Sudan. Available from http://www.who.int/csr/don/2004_08_10/en/. Accessed August 30, 2004.
  7. World Health Organization. Communicable Disease Surveillance and Response (CSR), Hepatitis E in Chad. Available from http://www.who.int/csr/don/2004_08_19/en/. Accessed August 30, 2004.
  8. International Herald Tribune. Hepatitis Spreads in 2 Iraqi Districts. Available from http://www.iht.com/articles/540294.html. Accessed September 27, 2004.
  9. Thomas DL, Yarbough PO, Vlahov D, Tsarev SA, Nelson KE, Saah AJ, Purcell RH, 1997. Seroreactivity to hepatitis E virus in areas where the disease is not endemic. J Clin Microbiol 35 : 1244–1247.
  10. Krawczynski K, 1993. Hepatitis E. Hepatology 17 : 932–941.
  11. Purdy MA, Carson D, McCaustland KA, Bradley DW, Beach MJ, Krawczynski K, 1994. Viral specificity of hepatitis E virus antigens identified by fluorescent antibody assay using recombinant HEV proteins. J Med Virol 44 : 212–214.
  12. Schlauder GG, Mushahwar IK, 2001. Genetic heterogeneity of hepatitis E virus. J Med Virol 65 : 282–292.
  13. Meng XJ, Purcell RH, Halbur PG, Lehman JR, Webb DM, Tsareva TS, Haynes JS, Thacker BJ, Emerson SU, 1997. A novel virus in swine is closely related to the human hepatitis E virus. Proc Natl Acad Sci USA 94 : 9860–9865.
  14. Wang Y, Zhang H, Ling R, Li H, Harrison TJ, 2000. The complete sequence of hepatitis E virus genotype 4 reveals an alternative strategy for translation of open reading frames 2 and 3. J Gen Virol 81 : 1675–1686.
  15. Aggarwal R, Krawczynski K, 2000. Hepatitis E: An overview and recent advances in clinical and laboratory research. Gastroenterol Hepatol 15 : 9–20.
  16. McCaustland KA, Bi S, Purdy MA, Bradley DW, 1991. Application of two RNA extraction methods prior to amplification of hepatitis E virus nucleic acid by the polymerase chain reaction. J Virol Methods 35 : 331–342.
  17. Erker JC, Desai SM, Mushahwar IK, 1999. Rapid detection of Hepatitis E virus RNA by reverse transcription-polymerase chain reaction using universal oligonucleotide primers. J Virol Methods 81 : 109–113.
  18. Tsarev SA, Binn LN, Gomatos PJ, Arthur RR, Monier MK, van Cuyck-Gandre H, Longer CF, Innis BL, 1999. Phylogenetic analysis of hepatitis E virus isolates from Egypt. J Med Virol 57 : 68–74.
  19. Seriwatana J, Shrestha MP, Scott RM, Tsarev SA, Vaughn DW, Myint KS, Innis BL, 2002. Clinical and epidemiological relevance of quantitating hepatitis E virus-specific immunoglobin M. Clin Diagn Lab Immunol 9 : 1072–1078.
  20. Clopper CJ, Pearson ES, 1934. The use of confidence or fiducial limits illustrated in the case of the binomial. Biometrika 26 : 404–413.
  21. Anderson DA, Li F, Riddell MA, Howard T, Seow H-F, Torresi J, Perry G, Sumarsidi D, Shrestha SM, Shrestha IL, 1999. ELISA for IgG-class antibody to hepatitis E virus based on a highly conserved, conformational epitope expressed in Escherichia coli. J Virol Methods 81 : 131–142.
  22. Riddell MA, Li F, Anderson DA, 2000. Identification of immunodominant and conformational epitopes in the capsid protein of hepatitis E virus by using monoclonal antibodies. J Virol 74 : 8011–8017.
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  • Received : 27 Jan 2005
  • Accepted : 19 May 2005

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