1921
Volume 73, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

To study gene expression differences between oocyst and salivary gland sporozoites, cDNA libraries previously constructed from the two sporozoite populations of the avian malaria parasite, , were used in a subtractive hybridization protocol to isolate , a novel oocyst sporozoite gene. encodes a putative ~76 kDa translated protein that was predicted to localize to the nucleus. Transcriptional analysis indicates that is preferentially expressed in oocyst sporozoites versus salivary gland sporozoites. Immunolocalization assays confirm both the nuclear prediction and transcriptional analysis, suggesting that is a nuclear protein. BLAST sequence analysis indicates that represents a novel gene that has significant homology with a hypothetical protein and translated and nucleotide sequences. This is the first characterization of a nuclear protein that shows preferential expression in one sporozoite population as compared with the other population.

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2005-09-01
2017-11-25
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  • Received : 19 Nov 2004
  • Accepted : 18 Mar 2005

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