1921
Volume 73, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Detection of malaria during pregnancy is complicated by sequestration of parasites in the placenta, which reduces peripheral blood microscopic detection. Laser desorption mass spectrometry (LDMS) has previously demonstrated sensitive detection of hemozoin from blood cultures and the ability to track parasitemia in a malaria mouse model. Here we use a simple, dilution in water, blood sample preparation protocol for LDMS detection of malaria in 45 asymptomatic, pregnant Zambian women. We compare LDMS to microscopy and polymerase chain reaction (PCR) analysis. All women were microscopy negative. LDMS detected hemozoin in 15 out of 45 women, while PCR results were positive in 25 women. Compared with PCR, which analyzed 20–30 μL of blood, the sensitivity of LDMS, which analyzed < 1 μL of blood, was 52%, with a specificity of 92%. LDMS is a potentially rapid and more sensitive alternate diagnostic method than microscopy.

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References

  1. ter Kuile FO, Parise ME, Verhoeff FH, Udhayakumar V, Newman RD, van Eijk AM, Rogerson SJ, Steketee RW, 2004. The burden of co-infection with human immunodeficiency virus type 1 and malaria in pregnant women in sub-saharan Africa. Am J Trop Med Hyg 71 : 41–54. [Google Scholar]
  2. Guyatt HL, Snow RW, 2004. Impact of malaria during pregnancy on low birth weight in sub-Saharan Africa. Clin Microbiol Rev 17 : 760–769. [Google Scholar]
  3. Duffy PE, Fried M, 2003. Plasmodium falciparum adhesion in the placenta. Curr Opin Microbiol 6 : 371–376. [Google Scholar]
  4. Hastings IM, Watkins WM, White NJ, 2002. The evolution of drug-resistant malaria: the role of drug elimination half-life. Philos Trans R Soc Lond B Biol Sci 357 : 505–519. [Google Scholar]
  5. van Eijk AM, Ayisi JG, ter Kuile FO, Otieno JA, Misore AO, Odondi JO, Rosen DH, Kager PA, Steketee RW, Nahlen BL, 2004. Effectiveness of intermittent preventive treatment with sulphadoxine-pyrimethamine for control of malaria in pregnancy in western Kenya: a hospital-based study. Trop Med Int Health 9 : 351–360. [Google Scholar]
  6. Kayentao K, Kodio M, Newman RD, Maiga H, Doumtabe D, Ongoiba A, Coulibaly D, Keita AS, Maiga B, Mungai M, Parise ME, Doumbo O, 2005. Comparison of intermittent preventive treatment with chemoprophylaxis for the prevention of malaria during pregnancy in Mali. J Infect Dis 191 : 109–116. [Google Scholar]
  7. Leke RF, Djokam RR, Mbu R, Leke RJ, Fogako J, Megnekou R, Metenou S, Sama G, Zhou Y, Cadigan T, Parra M, Taylor DW, 1999. Detection of the Plasmodium falciparum antigen histidine-rich protein 2 in blood of pregnant women: implications for diagnosing placental malaria. J Clin Microbiol 37 : 2992–2996. [Google Scholar]
  8. Moody A, 2002. Rapid diagnostic tests for malaria parasites. Clin Microbiol Rev 15 : 66–78. [Google Scholar]
  9. De Monbrison F, Gerome P, Chaulet JF, Wallon M, Picot S, Peyron F, 2004. Comparative diagnostic performance of two commercial rapid tests for malaria in a non-endemic area. Eur J Clin Microbiol Infect Dis 23 : 784–786. [Google Scholar]
  10. Sullivan DJ, 2002. Theories on malarial pigment formation and quinoline action. Int J Parasitol 32 : 1645–1653. [Google Scholar]
  11. Slomianny C, 1990. Three-dimensional reconstruction of the feeding process of the malaria parasite. Blood Cells 16 : 369–378. [Google Scholar]
  12. Scholl PF, Kongkasuriyachai D, Demirev PA, Feldman AB, Lin JS, Sullivan DJ Jr, Kumar N, 2004. Rapid detection of malaria infection in vivo by laser desorption mass spectrometry. Am J Trop Med Hyg 71 : 546–551. [Google Scholar]
  13. Demirev PA, 2004. Mass spectrometry for malaria diagnosis. Expert Rev Mol Diagn 4 : 821–829. [Google Scholar]
  14. Demirev PA, Feldman AB, Kongkasuriyachai D, Scholl P, Sullivan D Jr, Kumar N, 2002. Detection of malaria parasites in blood by laser desorption mass spectrometry. Anal Chem 74 : 3262–3266. [Google Scholar]
  15. Trager W, Jensen JB, 1976. Human malaria parasites in continuous culture. Science 193 : 673–675. [Google Scholar]
  16. Lambros C, Vanderberg JP, 1979. Synchronization of Plasmodium falciparum erythrocytic stages in culture. J. Parasitol. 65 : 418–420. [Google Scholar]
  17. Plowe CV, Djimde A, Bouare M, Doumbo O, Wellems TE, 1995. Pyrimethamine and proguanil resistance-conferring mutations in Plasmodium falciparum dihydrofolate reductase:polymerase chain reaction methods for surveillance in Africa. Am. J. Trop. Med. Hyg. 52 : 565–568. [Google Scholar]
  18. Duraisingh MT, Jones P, Sambou I, von Seidlein L, Pinder M, Warhurst DC, 2000. The tyrosine-86 allele of the pfmdr1 gene of Plasmodium falciparum is associated with increased sensitivity to the anti-malarials mefloquine and artemisinin. Mol Biochem Parasitol 108 : 13–23. [Google Scholar]
  19. Duraisingh MT, Curtis J, Warhurst DC, 1998. Plasmodium falciparum: detection of polymorphisms in the dihydrofolate reductase and dihydropteroate synthetase genes by PCR and restriction digestion. Exp Parasitol 89 : 1–8. [Google Scholar]
  20. Djimde A, Doumbo OK, Cortese JF, Kayentao K, Doumbo S, Diourte Y, Dicko A, Su XZ, Nomura T, Fidock DA, Wellems TE, Plowe CV, Coulibaly D, 2001. A molecular marker for chloroquine-resistant falciparum malaria. N Engl J Med 344 : 257–263. [Google Scholar]
  21. Feldman AB, Lin JS, Demirev PA, Pisciotta J, Haddad D, Scholl P, Sullivan JD, Kumar N, Kongkasuriyachai D, Miller RS, Gasser RA, 2003. A novel method for rapid and affordable malaria screening. 52nd Annual Meeting of the American Society of Tropical Medicine and Hygiene. Philadelphia Marriott Downtown Hotel, Philadelphia, Pennsylvania: Am J Trop Med Hyg, Late breaker.
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  • Received : 25 Jan 2005
  • Accepted : 06 Apr 2005

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