Volume 73, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


In response to recent increases in cases of pediatric encephalitis with unknown etiology in northern Vietnam, surveillance for arbovirus activity was conducted in four provinces surrounding the city of Hanoi during June 2002 and July–August 2004. A total of 20,615 mosquitoes consisting of 19 species in 1,122 pools were processed for virus isolation; virus isolates were obtained from 44 pools. (11 isolates), (15 isolates), (13 isolates), and (4 isolates) were identified by immunofluorescence assay and sequence analysis of reverse transcription–polymerase chain reaction fragments. Surprisingly, no isolates of Japanese encephalitis (JE) virus were obtained. Isolation of and is reported for the first time from Vietnam.


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  1. National Institute of Hygiene and Epidemiology, 2003. Annual Epidemiological Report on Emerging, Re-Emerging and Communicable Diseases in Vietnam. Hanoi: Department of Epidemiology, National Institute of Hygiene and Epidemiology.
  2. Solomon T, Dung NM, Kneen R, Thao LTT, Gainsborough M, Nisalak A, Day NPJ, Kirkam FJ, Vaughn DW, Smith S, White N, 2002. Seizures and raised intracranial pressure in Vietnamese patients with Japanese encephalitis. Brain 125 : 1084–1093.
    [Google Scholar]
  3. Stojanovich CJ, Scott HG, 1966. Illustrated Key to Mosquitoes of Vietnam. Atlanta, GA: U.S. Department of Health, Education, and Welfare, Public Health Service.
  4. Reuben R, Tewari SC, Hiriyan J, Akiyama J, 1994. Illustrated keys to species of Culex (Culex) associated with Japanese encephalitis in southeast asia (Diptera: culicidae). Mosq Systematics 26 : 75–96.
    [Google Scholar]
  5. Miller BR, Mitchell CJ, Ballinger ME, 1989. Replication, tissue tropisms and transmission of yellow fever virus in Aedes albopictus. Trans R Soc Trop Med Hyg 83 : 252–255.
    [Google Scholar]
  6. Tsai TF, 1995. Arboviruses. Murray PR, ed. Manual of Clinical Microbiology. Washington, DC: American Society of Microbiology, 980–996.
  7. Kuno G, Chang GJ, Tsuchiya KR, Karabatsos N, Cropp CB, 1998. Phylogeny of the genus Flavivirus. J Virol 72 : 73–83.
    [Google Scholar]
  8. Powers AM, Brault AC, Shirako Y, Strauss EG, Kang W, Strauss JH, Weaver SC, 2001. Evolutionary relationships and systematics of the alphaviruses. J Virol 75 : 10118–10131.
    [Google Scholar]
  9. Kono Y, Yusnita Y, Mohn Ali AR, Maizan M, Sharifar SH, Fauzia O, Kubo M, Aziz AJ, 2002. Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection. Arch Virol 147 : 1623–1630.
    [Google Scholar]
  10. Shirako Y, Yamaguchi Y, 2000. Genome structure of Sagiyama virus and its relatedness to other alphaviruses. J Gen Virol 81 : 1353–1360.
    [Google Scholar]
  11. Gatton ML, Kelly-Hope LA, Kay BH, Ryan PA, 2004. Spatial-temporal analysis of Ross River virus disease patterns in Queensland, Australia. Am J Trop Med Hyg 71 : 629–635.
    [Google Scholar]
  12. Huang C-C, Huang T-S, Deng M-C, Jong M-H, Lin S-Y, 2003. Natural infections of pigs with Akabane virus. Vet Microbiol 94 : 1–11.
    [Google Scholar]
  13. Hamana K, 2001. Kagoshima University Research Center for the Pacific Islands. Occasional Papers. 4 : 163–167.
    [Google Scholar]
  14. Stram Y, Brenner J, Braverman Y, Banet-Noach C, Kuznetzova L, Ginni M, 2004. Detection and quantitation of Akabane and Aino viruses by multiplex real-time reverse transcriptase PCR. J Virol Methods 116 : 147–154.
    [Google Scholar]

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  • Received : 26 Jan 2005
  • Accepted : 13 Mar 2005
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