Volume 73, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


The purpose of this study was to determine whether HIV-1 infected patients in our community were more susceptible to and infection than non-HIV-infected individuals. The prevalence and frequency of invasive amebiasis was determined in 203 HIV/AIDS subjects and 140 close relatives or sexual partners, all of whom were HIV. Anti– antibodies (IgG, IgA) were assessed as indicators of invasive infection. Polymerase chain reaction (PCR) was used for the characterization of the species. The prevalence estimated with PCR data showed that infection was more common in the HIV/AIDS group (25.32%), than in HIV contacts (18.46%). + infection was more frequent in HIV/AIDS patients (13.3%), than in HIV contacts (0.7%). and/or infection was highly prevalent in HIV/AIDS patients (34.1%) without evidence of recent or current invasive disease. Contacts of HIV/AIDS patients who were infected with were asymptomatic cyst passers. Our results suggest that strains prevalent in the studied community appear to be of low pathogenic potential.


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  1. Smith PD, Lane HC, Gill VJ, Manischewitz JF, Quinnan GN, Fauci AS, Masur H, 1988. Intestinal infections in patients with acquired immunodeficiency syndrome (AIDS). Ann Intern Med 108 : 328–333.
  2. Loughton BE, Druckman DA, Vernon A, Quinn TC, Polk BP, 1988. Prevalence of enteric pathogens in homosexual men with and without acquired immunodeficiency syndrome. Gastroenterology 94 : 948–993.
  3. Dupont HL, Marshall GD, 1995. HIV-associated diarrhea and wasting. Lancet ii : 352–356.
  4. Hung CC, Chen PS, Hsieh SM, Wong JM, Fang CT, Chang SC, Chen MY, 1999. Invasive amoebiasis: an emerging parasitic disease in patients infected with HIV in an area endemic for amoebic infection. AIDS 13 : 2421–2428.
  5. Fontanet AL, Sahlu T, Rinkedewit T, Messele T, Masho W, Woldemichael T, Yeneneh H, Coutinho RA, 2000. Epidemiology of infections with intestinal parasites and human immunodeficiency virus (HIV) among Sugar-State residents in Ethiopia. Ann Trop Med Parasitol 94 : 269–278.
  6. Actor JK, Shirai M, Kullberg MC, Buller RM, Sher A, Berzofsky JA, 1993. Helminth infection results in decreased virus-specific CD8+ cytotoxic T-cells and Th1 cytokine responses as well as delayed virus clearance. Proc Natl Acad Sci USA 90 : 948–952.
  7. Bentwich Z, Kalinkovich A, Weisman Z, 1995. Immune activation in a dominant factor in the pathogenesis of African AIDS. Immunol Today 16 : 187–191.
  8. Rizzardini G, Piconi S, Ruzzante S, Fusi ML, Lukwiya M, Declich S, Tamburini M, Villa ML, Fabiani M, Milazzo F, Clerici M, 1996. Immunological activation marker in the serum of African and European HIV-seropositive and seronegative individuals. AIDS 10 : 1535–1542.
  9. Salata RA, Ravdin JL, 1986. Review of human immune mechanisms directed against Entamoeba histolytica. Rev Infect Dis 8 : 261–272.
  10. Takeuchi T, Miyahira Y, Kobayashi S, Nozaki T, Mottha S, Matsuda J, 1990. High seropositivity for Entamoeba histolytica in Japanese homosexual men: further evidence for the occurrence of pathogenic strains. Am J Trop Med Hyg 84 : 250–251.
  11. Reed SL, Wessed DW, David CE, 1991. Entamoeba histolytica infection and AIDS. Am J Med 90 : 269–271.
  12. Ramos F, Valdez E, Morán P, González E, Padilla G, Gómez A, Ramiro M, Melendro EI, Muñoz O, Clark G, Ximénez C, 2000. Prevalence of Entamoeba histolytica and Entamoeba dispar in highly endemic rural population. Arch Med Res 31 : 34–35.
  13. Caballero A, Viveros RM, Salvatierra B, Tapia CR, Sepúlveda J, Gutiérrez G, Ortíz OL, 1994. Seroepidemiology of amebiasis in Mexico. Am J Trop Med Hyg 50 : 412–419.
  14. Quinn TC, Stamm WE, Goodell SE, 1983. The polymicrobial origin of intestinal infection in homosexual men. N Engl J Med 309 : 576–582.
  15. Bartlett JG, 1999. The Johns Hopkins Hospital 1998–1999 Guide to Medical Care of Patients with HIV Infection. Baltimore, MD: Williams and Wilkins.
  16. Investigación en humanos, 2003. Ley General de Salud. Gobierno Federal de los Estados Unidos Mexicanos. SISTA editor, Mexico D.F. pp. 32–34.
  17. Ash RL, Orihel TC, 1987. Collection and preservation of faeces. American Society of Clinical Pathologists, eds. Parasites: A Guide to Laboratory Procedures and Identification. Chicago, IL: ASCP Press, 5–14.
  18. Acuña-Soto R, Samuelson J, De Girolami P, Zárate L, Millan-Velasco F, Schoolmick G, Wirth D, 1993. Application of the polymerase chain reaction to the epidemiology of pathogenic and non-pathogenic Entamoeba histolytica. Am J Trop Med Hyg 48 : 58–70.
  19. Clark GC, Diamond LS, 1992. Differentiation of pathogenic Entamoeba histolytica from other intestinal protozoa by riboprinting. Arch Med Res 23 : 15–18.
  20. Strauss K, Hannet I, Engels S, 1996. Performance evaluation of the FACS Court System: a dedicated system for clinical cellular analysis. Cytometry 26 : 52–59.
  21. Valenzuela O, Ramos F, Morán P, González E, Valadez A, Gómez A, Melendro EI, Ramiro M, Muñoz O, Ximénez C, 2001. Persistence of secretory antiamoebic antibodies in patients with invasive intestinal or hepatic amoebiasis. Parasitol Res 87 : 849–852.
  22. Kleinbaum DG, Kupper LL, Morgenster H, 1982. Measures of potential impact and summary of the measures. Kleinbaun DG, ed. Epidemiological Research. Principles and Quantitative Methods. New York: Van Nostrand Reinhold, 159–180.
  23. Germani Y, Minssart P, Vohito M, Yassibanda S, Gaziou P, Hocquet D, Berthélémy P, Morvan J, 1998. Etiologies of acute, persistent, and dysenteric diarrheas in adults in Bangui, Central African Republic, in relation to human immunodeficiency virus serostatus. Am J Trop Med Hyg 59 : 1008–1014.
  24. Waywa D, Kongkriengdaj S, Chaidatch S, Tiengrim S, Kowadisaiburana B, Chaikachonpat S, Suwanagool S, Chaiprasert A, Curry A, Bailey W, Suputtamongkol Y, Beeching NJ, 2001. Protozoan enteric infection in AIDS related diarrhea in Thailand. South East Asian. J Trop Med Public Health 32 : 151–155.
  25. Olmos M, Molina C, Piskorz E, Tawil J, Magnanini F, Casiro A, Cahn P, Concetti H, Janeiro M, Guelfand L, Kaufman S, 1996. Diahrrea and AIDS: more complex diagnostic techniques; better therapeutic results. Acta Gastroenterol Latinoam 26 : 91–100.
  26. Joshi M, Chowdhary AS, Dalal PJ, Manar JK, 2002. Parasitic diarrhoea in patients with AIDS. Nat Med J India 15 : 72–74.
  27. Borgdorff M, 1994. Epidemiology of HIV-1 infection in Mwanza region, Tanzania. Thesis, Royal Tropical Institute, Amsterdam, The Netherlands.
  28. Smith PD, Lane HC, Gill VJ, Manischewitz JF, Quinnan GV, Fauci AS, Masur H, 1998. Intestinal infections in patients with the acquired immunodeficiency syndrome (AIDS). Ann Int Med 108 : 328–333.
  29. Lowther SA, Dworkin MS, Hanson DL, 2000. Entamoeba histolytica/Entamoeba dispar infections in human immunodeficiency virus infected patients in the United States. Clin Infect Dis 30 : 955–959.
  30. Esfandiari A, Jordan WC, Brown CP, 1995. Prevalence of enteric parasitic infection among HIV-infected attendees of an inner city AIDS clinic. Cell Mol Biol 41 : 519–523.
  31. Braga LL, Mendonca Y, Paiva CA, Sales A, Cavalcante ALM, Mann BJ, 1998. Seropositivity for intestinal colonization with Entamoeba histolytica and Entamoeba dispar in individuals in Northeastern Brazil. J Clin Microbiol 36 : 3044–3045.
  32. Haghighi A, Kobayashi S, Takeuchi T, Masuda G, Nosaki T, 2002. Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geographic area. J Clin Microbiol 40 : 4081–4090.
  33. Bhattacharya S, Bhattacharya A, Diamond LS, 1992. Entamoeba histolytica extrachromosomal circular ribosomal DNA: analysis of clonal variation in a hypervariable region. Exp Parasitol 74 : 200–204.
  34. Clark CG, Diamond LS, 1993. Entamoeba histolytica: a method for isolated identification. Exp Parasitol 77 : 450–455.
  35. Zabi J, Clark CG, 2001. Isolation and characterization of polymorphic DNA from Entamoeba histolytica. J Clin Microbiol 39 : 897–905.
  36. Li E, Kunz-Jenkins C, Stanley SL, 1992. Isolation and characterization of genomic clones encoding a serine-rich Entamoeba histolytica protein. Mol Biochem Parasitol 50 : 355–357.
  37. Kholer S, Tannich E, 1993. A family of transcripts (K2) of Entamoeba histolytica polymorphic repetitive regions with highly conserved elements. Mol Biochem Parasitol 59 : 49–58.
  38. Burch DJ, Li E, Reed S, Jackson TF, Stanley SL, 1991. Isolation of a strain specific Entamoeba histolytica cDNA clone. J Clin Microbiol 29 : 696–701.
  39. Ghosh S, Frisardi M, Ramírez L, Descateaux S, Sturm K, Newton OA, Dantos JI, Ganguly CH, Lohia A, Reed S, Samuelson J, 2000. Molecular epidemiology of Entamoeba spp.: evidence of a bottleneck (demographic sweep) and transcontinental spread of diploid parasites. J Clin Microbiol 38 : 3815–3821.
  40. Ramiro M, Morán P, García G, Ramos F, Valadez A, González E, Melendro EI, Ximénez C, 2003. Coinfección por diferentes clonas de amiba, pertenecientes a la misma especie, en el humano. Medicina Interna de Mexico 19 : (S-1) 40.

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Supplementary Data

Notice of Inadvertent Duplicate Publication

  • Received : 14 Oct 2003
  • Accepted : 08 Feb 2005

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