1921
Volume 73, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Clinical isolates of from visceral leishmaniasis (VL) cases in Nepal and from cutaneous leishmaniasis (CL) cases in Peru, were cultured using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to type species and strain. Promastigotes from 38 isolates, within eight passages from isolation, were used to infect mouse peritoneal macrophage cultures and the amastigote sensitivity to miltefosine was determined. The concentration required to kill 50% of intracellular amastigotes from Nepalese VL isolates, all typed as ( = 24) from both Sb responders and nonresponders, ranged from 8.7 to 0.04 μg/mL. In contrast, the concentration required to kill 50% intracellular amastigotes from isolates from Peru, typed as ( = 8), was > 30 to 8.4 μg/mL, ( = 2) > 30 to 1.9 μg/mL, ( = 1) > 30 μg/mL, and ( = 4) was 3.4 to 1.9 μg/mL. This demonstrates a notable difference in the intrinsic sensitivity of species to miltefosine . If this model can be correlated to therapeutic outcome, it may have implications for the interpretation of clinical trials.

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2017-09-21
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  • Received : 29 Jun 2004
  • Accepted : 11 Feb 2005

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