1921
Volume 73, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The informative value of genotyping populations in single blood samples was studied before and during treatment in 13 patients with malaria. Genotyping of the two merozoite surface proteins ( [block 2] and ) and the glutamate-rich protein showed multiple genotypes in seven patients, and single genotypes in the remaining six patients. The same genotype profiles were detected in consecutive samples obtained every 12 hours during treatment from the respective patients, although some genotypes were cleared earlier than others. These patterns are in contrast to the extensive daily dynamics previously described in asymptomatic infections. The genotypes detected in one pre-treatment sample thus appear to reflect the parasite subpopulations of the clinical malaria infection during the following days, and additional sampling does not provide any additional information.

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2005-07-01
2017-11-24
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References

  1. World Health Organization, 2002. Monitoring Antimalarial Drug Resistance: Report of a WHO Consultation. Geneva: World Health Organization. December 3–5, 2001. WHO/CDS/CSR/EPH/2002.17.
  2. Snounou G, Beck HP, 1998. The use of PCR genotyping in the assessment of recrudescence or reinfection after antimalarial drug treatment. Parasitol Today 14 : 462–467.
  3. Magesa SM, Mdira KY, Farnert A, Simonsen PE, Bygbjerg IC, Jakobsen PH, 2001. Distinguishing Plasmodium falciparum treatment failures from re-infections by using polymerase chain reaction (PCR) genotyping in an holoendemic area, northeastern Tanzania. Am J Trop Med Hyg 65 : 477–483.
  4. Daubersies P, Sallenave-Sales S, Magne S, Trape JF, Contamin H, Fandeur T, Rogier C, Mercereau-Puijalon O, Druilhe P, 1996. Rapid turn over of Plasmodium falciparum populations in asymptomatic individuals living in a high transmission area. Am J Trop Med Hyg 54 : 18–26.
  5. Färnert A, Snounou G, Rooth I, Björkman A, 1997. Daily dynamics of Plasmodium falciparum subpopulations in asymptomatic children in a holoendemic area. Am J Trop Med Hyg 56 : 538–547.
  6. Bruce MC, Galinski MR, Barnwell JW, Donnelly CA, Walmsley M, Alpers MP, Walliker D, Day KP, 2000. Genetic diversity and dynamics of Plasmodium falciparum and P. vivax populations in multiply infected children with asymptomatic malaria infections in Papua New Guinea. Parasitology 121 : 257–272.
  7. Druilhe P, Daubersies P, Patarapotikul J, Gentil C, Chene L, Chongsuphajaisiddhi T, Mellouk S, Langsley G, 1998. A primary malarial infection is composed of a very wide range of genetically diverse but related parasites. J Clin Invest 10 : 2008–2016.
  8. Färnert A, Tengstam K, Palme IB, Bronner U, Lebbad M, Swedberg G, Bjorkman A, 2002. Polyclonal Plasmodium falciparum malaria in travelers and selection of antifolate mutations after proguanil prophylaxis. Am. J Trop Med Hyg 66 : 487–491.
  9. Snounou G, Viriyakosol S, Jarra W, Thaithong S, Brown KN, 1993. Identification of the four human parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections. Mol Biochem Parasitol 58 : 283–292.
  10. Snounou G, Zhu X, Siripoon N, Jarra W, Thaithong S, Brown KN, Viriyakosol S, 1999. Biased distribution of msp1 and msp2 allelic variants in Plasmodium falciparum populations in Thailand. Trans R Soc Trop Med Hyg 93 : 369–374.
  11. Jafari S, Le Bras J, Bouchaud O, Durand R, 2004. Plasmodium falciparum clonal population dynamics during malaria treatment. J Infect Dis 189 : 195–203.
  12. James SP, Nicol WD, Shute PG, 1936. Clinical and parasitological observation on induced malaria. Proc R Soc Med 29 : 879–894.
  13. Collins WE, Jeffery GM, 1999. A retrospective examination of secondary sporozoite- and trophozoite-induced infections with Plasmodium falciparum: development of parasitologic and clinical immunity following secondary infection. Am J Trop Med Hyg 61 (Suppl): 20–35.
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  • Received : 18 Oct 2004
  • Accepted : 15 Dec 2004

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