Volume 73, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


After a trip to Zambia, a previously healthy adult traveler presented with a prolonged illness characterized by low-grade fevers and fatigue. Although malaria smears and antibody tests results for species were negative, a diagnosis of malaria was ultimately determined by polymerase chain reaction (PCR) amplification and species-specific nucleic acid hybridization techniques. The patient was successfully treated and cured. Clinical use of PCR technology may facilitate the identification of cases of smear-negative malaria, which up to the present time, have been difficult to diagnose.


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  1. Mehlotra RK, Kasehagen LJ, Baisor M, Lorry K, Kazura JW, Bockarie MJ, Zimmerman PA, 2002. Malaria infections are randomly distributed in diverse holoendemic areas of Papua New Guinea. Am J Trop Med Hyg 67 : 555–562.
    [Google Scholar]
  2. Williams HA, Roberts J, Kachur SP, Barber AM, Barat LM, Bloland PB, Ruebush TK, Wolfe EB, 1999. Malaria surveillance—United States, 1995. MMWR CDC Surveill Summ 48 : 1–23.
    [Google Scholar]
  3. Procop GW, Persing DH, 2001. Malaria and Babesia. Wilson WR, Sande MA, eds. Current Diagnosis and Treatment in Infectious Diseases. New York: McGraw-Hill, 793–806.
  4. White NJ, 1996. The treatment of malaria. N Engl J Med 335 : 800–806.
    [Google Scholar]
  5. Looareesuwan S, Chulay JD, Canfield CJ, Hutchinson DB, 1999. Malarone (atovaquone and proguanil hydrochloride): a review of its clinical development for treatment of malaria. Malarone Clinical Trials Study Group. Am J Trop Med Hyg 60 : 533–541.
    [Google Scholar]
  6. Tham JM, Lee SH, Tan TMC, Ting RCY, Kara UAK, 1999. Detection and species determination of malaria parasites by PCR: comparison with microscopy and with Parasight-F and ICT malaria Pf tests in a clinical environment. J Clin Microbiol 37 : 1269–1273.
    [Google Scholar]
  7. Postigo M, Mendoza-Leon A, Perez HA, 1998. Malaria diagnosis by the polymerase chain reaction: a field study in southeastern Venezuela. Trans R Soc Trop Med Hyg 92 : 509–511.
    [Google Scholar]
  8. Hanscheid T, Grobusch M, 2002. How useful is PCR in the diagnosis of malaria? Trends Parasitol 18 : 395–398.
    [Google Scholar]
  9. Farcas GA, Zhong KJY, Mazzulli T, Kain KC, 2004. Evaluation of the RealArt malaria LC real-time PCR assay for malaria diagnosis. J Clin Microbiol 42 : 636–638.
    [Google Scholar]
  10. de Monbrison F, Angei C, Staal A, Kaiser K, Picot S, 2003. Simultaneous identification of the four human Plasmodium species and quantification of Plasmodium DNA load in human blood by real-time polymerase chain reaction. Trans R Soc Trop Med Hyg 97 : 387–390.
    [Google Scholar]
  11. Kain KC, Keystone JS, 1998. Malaria in travelers. Epidemiology, disease, and prevention. Infect Dis Clin North Am 12 : 267–284.
    [Google Scholar]

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  • Received : 10 Sep 2004
  • Accepted : 08 Feb 2005
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