1921
Volume 73, Issue 5_suppl
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The merozoite surface protein 1 (MSP-1) is expressed in all species and is considered a major malaria vaccine candidate. We found that MSP-1 from (MSP-1) contains a region of significant sequence homology with the 190L subunit vaccine derived from the MSP-1. The fragment, termed 200L, was expressed as a recombinant protein in (r200L) and used to asses its immunologic relevance as a vaccine target. A cross-sectional, seroepidemiologic study conducted in Buenaventura, Colombia showed that 52.2% (95% confidence interval [CI] = 39.8–64.3) of individuals previously exposed to and 72.8% (95% CI = 61.8–82.1) of –infected patients had IgG antibodies to r200L. Immunization of BALB/c mice and monkeys induced IgG antibodies (titer > 10) that cross-reacted with parasites. Immunized monkeys displayed partial protection against a challenge with blood stages. Our results suggest that 200L is a new malaria vaccine subunit and deserves further testing.

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  • Received : 13 May 2005
  • Accepted : 29 Jul 2005

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