1921
Volume 73, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The diagnostic potential of a type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65% and the specificity was 100%, with no PCR-positive results in specimens from 18 patients without melioidosis. The PCR based on the TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis.

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References

  1. White NJ, 2003. Melioidosis. Lancet 361 : 1715–1722. [Google Scholar]
  2. Cheng AC, Currie BJ, 2005. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18 : 383–416. [Google Scholar]
  3. Chaowagul W, White NJ, Dance DA, Wattanagoon Y, Naigowit P, Davis TM, Looareesuwan S, Pitakwatchara N, 1989. Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand. J Infect Dis 159 : 890–899. [Google Scholar]
  4. Dance DA, Wuthiekanun V, Naigowit P, White NJ, 1989. Identification of Pseudomonas pseudomallei in clinical practice: use of simple screening tests and API 20NE. J Clin Pathol 42 : 645–648. [Google Scholar]
  5. Lew AE, Desmarchelier PM, 1994. Detection of Pseudomonas pseudomallei by PCR and hybridization. J Clin Microbiol 32 : 1326–1332. [Google Scholar]
  6. Dharakul T, Songsivilai S, Viriyachitra S, Luangwedchakarn V, Tassaneetritap B, Chaowagul W, 1996. Detection of Burkholderia pseudomallei DNA in patients with septicemic melioidosis. J Clin Microbiol 34 : 609–614. [Google Scholar]
  7. Kunakorn M, Markham RB, 1995. Clinically practical seminested PCR for Burkholderia pseudomallei quantitated by enzyme immunoassay with and without solution hybridization. J Clin Microbiol 33 : 2131–2135. [Google Scholar]
  8. Rattanathongkom A, Sermswan RW, Wongratanacheewin S, 1997. Detection of Burkholderia pseudomallei in blood samples using polymerase chain reaction. Mol Cell Probes 11 : 25–31. [Google Scholar]
  9. Tomaso H, Pitt TL, Landt O, Dahouk SA, Scholz HC, Reisinger EC, Sprague LD, Rathmann I, Neubauer H, 2005. Rapid presumptive identification of Burkholderia pseudomallei with real-time PCR assays using fluorescent hybridization probes. Mol Cell Probes 19 : 9–20. [Google Scholar]
  10. Kunakorn M, Raksakait K, Sethaudom C, Sermswan RW, Dharakul T, 2000. Comparison of three PCR primer sets for diagnosis of septicemic melioidosis. Acta Trop 74 : 247–251. [Google Scholar]
  11. Haase A, Brennan M, Barrett S, Wood Y, Huffam S, O’Brien D, Currie B, 1998. Evaluation of PCR for diagnosis of melioidosis. J Clin Microbiol 36 : 1039–1041. [Google Scholar]
  12. Sermswan RW, Wongratanacheewin S, Anuntagool N, Sirisinha S, 2000. Comparison of the polymerase chain reaction and serologic tests for diagnosis of septicemic melioidosis. Am J Trop Med Hyg 63 : 146–149. [Google Scholar]
  13. Winstanley C, Hales BA, Hart CA, 1999. Evidence for the presence in Burkholderia pseudomallei of a type III secretion system-associated gene cluster. J Med Microbiol 48 : 649–656. [Google Scholar]
  14. Winstanley C, Hart CA, 2000. Presence of type III secretion genes in Burkholderia pseudomallei correlates with Ara(-) phenotypes. J Clin Microbiol 38 : 883–885. [Google Scholar]
  15. Smith-Vaughan HC, Gal D, Lawrie PM, Winstanley C, Sriprakash KS, Currie BJ, 2003. Ubiquity of putative type III secretion genes among clinical and environmental Burkholderia pseudomallei isolates in northern Australia. J Clin Microbiol 41 : 883–885. [Google Scholar]
  16. Walsh AL, Smith MD, Wuthiekanun V, Supputamongkol Y, Chaowagul W, Dance DA, Angus B, White NJ, 1995. Prognostic significance of quantitative bacteremia in septicemic melioidosis. Clin Infect Dis 21 : 1498–1500. [Google Scholar]
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  • Received : 24 Jun 2005
  • Accepted : 10 Aug 2005

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