Volume 73, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


The diagnostic potential of a type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65% and the specificity was 100%, with no PCR-positive results in specimens from 18 patients without melioidosis. The PCR based on the TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis.


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  • Received : 24 Jun 2005
  • Accepted : 10 Aug 2005

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