Volume 73, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


This paper reports the results of serologic, structural, biochemical, and genetic studies indicating that Araguari virus, a previously unassigned viral agent, is a member of the family Orthomyxoviridae and genus Araguari virus has six RNA fragments; biologically, it shares several properties with other viruses in the family Orthomyxoviridae. Nucleotide sequencing of the RNA segments 4 (glycoprotein) and 5 (nucleoprotein) of Araguari virus aligned with the orthomyxoviruses, showing the closest relationship with Thogoto virus (sequence similarity = 61.9% and 69.1%, respectively, for glycoprotein and nucleoprotein), but also sharing a more distant similarity with Dhori and Influenza C viruses, especially for the glycoprotein gene. Based on these results, we propose that Araguari virus should be assigned as a new member of the family and genus .


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  1. Karabatsos N, 1985. International Catalog of Arboviruses, Including Certain other Viruses of Vertebrates. Third edition. San Antonio, TX: American Society for Tropical Medicine and Hygiene, 33–34.
  2. Zeller HG, Karabatsos N, Calisher CH, Digoutte JP, Cropp CB, Murphy FA, Shope RE, 1989. Electron microscopic and antigenic studies of uncharacterized viruses. I. Evidence suggesting the placement of viruses in families Arenaviridae, Paramyxoviridae, or Poxviridae. Arch Virol 108 : 191–209. [Google Scholar]
  3. Calisher CH, Carstens EB, Christian P, Mahy BWJ, Mayo MA, Shope RE, 2000. Unassigned viruses. van Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB, eds. Virus Taxonomy, Classification and Nomenclature of Viruses. Seventh Report of the International Committee on Taxonomy of Viruses, San Diego, CA: Academic Press, 995–1008.
  4. Beaty B, Calisher CH, Shope RE, 1995. Arboviruses. Lennette DA, ed. Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, Washington, DC: American Public Health Association, 797–855.
  5. Clarke DH, Casals J, 1958. Techinique for hemagglutination and hemagglutination inhibition with arthropod-borne viruses. Am J Trop Med Hyg 7 : 561–573. [Google Scholar]
  6. Hsiung GD, Fong CKY, August MJ, 1979. The use of electron microscopy for diagnosis of virus infections: an overview. Prog Med Virol 25 : 133–159. [Google Scholar]
  7. Almeida JD, Waterson AP, 1969. The morphology of virus-antibody interaction. Adv Virus Res 15 : 307–338. [Google Scholar]
  8. Almeida JD, 1980. Practical aspects of diagnostic electron microscopy. Yale J Biol Med 53 : 5–18. [Google Scholar]
  9. Araújo MTF, Araújo R, Oliveira ML, 1979. Processamento de tecidos para análise da ultra-estrutura celular. Hileia Med 1 : 13–15. [Google Scholar]
  10. Luft JH, 1961. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 2 : 409–414. [Google Scholar]
  11. Reynolds ES, 1963. The use of lead citrate and high pH as an electron opaque stain in electron microscopy. J Cell Biol 17 : 208–213. [Google Scholar]
  12. Bullock GR, Petrusz P, 1982. Techiniques in Immunocytochemistry. London: Academic Press, 34–55.
  13. Bozzola JJ, Russell LD, 1999. Electron Microscopy: Principles and Techniques for Biologists. Second edition. Boston: Jones and Bartlett Publishers.
  14. Rico-Hesse R, Pallansh MA, Mottay BK, Kew OM, 1987. Geographic distribuition of wild poliovirus type 1 genotypes. Virology 160 : 311–322. [Google Scholar]
  15. Frese M, Weber M, Weber F, Speth V, Haller O, 1997. Mx1 sensitivity: Batken virus is an orthomyxovirus closely related to Dhori virus. J Gen Virol 78 : 2453–2458. [Google Scholar]
  16. Saitou N, Nei M, 1987. The neighbor-joining method: a new method for reconstruction phylogenetic trees. Mol Biol Evol 4 : 406–425. [Google Scholar]
  17. Swofford DL, 1998. PAUP. Phylogenetic Analysis Using Parsimony (and Other Methods). Version 4. Suderland, MA: Sinauer Associates.
  18. Kumar S, Tamura K, Nei M, 2000. Molecular Evolutionary Genetic Analysis. Version 1.01. University Park, PA: Pennsylvania State University.
  19. Kimura M, 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J Gen Virol 77 : 1761–1768. [Google Scholar]
  20. Felsenstein J, 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 : 783–791. [Google Scholar]
  21. Cox NJ, Fuller F, Kaverin N, Klenk HD, Lamb RA, Mahy BWJ, McCauley J, Nakamura K, Palese P, Webster R, 2000. Family Orthomyxoviridae. Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB, eds. Virus Taxonomy, Classification and Nomenclature. Seventh Report of the International Committee on Taxonomy of Viruses. San Diego, CA: Academic Press, 585–597.
  22. Dias LB, 1982. Histopatologia das Arboviroses A mazônicas. Simpósio Internacional sobre Arbovírus dos Trópicos e Febres Hemorrágicas. Rio de Janeiro, Brazil: Academia Brasileira de Ciências, 171–174.
  23. Dias LB, 1986. Patologia natural e experimental de arbovírus e vírus correlatos isolados na Amazônia. Ciências Biológicas e à Medicina Tropical. Instituto Evandro Chagas: 50 anos de contribuição às Fund Serv Saude Publica 1 : 439–450. [Google Scholar]
  24. Davies MCR, Jones LD, Nuttall PA, 1986. Experimental studies on the transmission cycle of Thogoto virus, a candidate orthomyxovirus, in Ricephalus appendiculatus. Am J Trop Med Hyg 49 : 1256–1262. [Google Scholar]
  25. Filipe AR, Casals J, 1979. Isolation of Dhori virus from Hyalomma marginatum ticks in Portugal. Intervirology 11 : 124–127. [Google Scholar]
  26. Filipe AR, Peleteiro MC, Monath TP, Calisher CH, 1986. Pathological lesions in mice infected with Thogoto virus, a tick-borne Orthomyxovirus. Acta Virol 30 : 337–340. [Google Scholar]
  27. Filipe AR, Peleteiro MC, Andrade HR, 1990. Dhori virus induced lesions in mice. Acta Virol 24 : 578–581. [Google Scholar]
  28. Both TF, Davies CR, Jones LD, Staunton D, Nuttall PA, 1989. Anatomical basis of Thogoto virus infection in BHK cell culture and in the ixodid tick vector, Rhipicephalus appendiculatus. J Gen Virol 70 : 1093–1104. [Google Scholar]
  29. Clouthier SC, Rector T, Brown NE, Anderson ED, 2002. Genomic organization of infectious salmon anaemia virus. J Gen Virol 83 : 421–428. [Google Scholar]
  30. Murphy FA, Webster RG, 1996. Orthomyxoviruses. Fields BN, Knipe DM, Howley PM, eds. Virology. Second edition. New York: Raven Press, 1091–1152.
  31. Stauton D, Nuttall PA, Bishop DH, 1989. Sequence analyses of Thogoto viral RNA segment 3: evidence for a distant relationship between members of the Orthomyxoviridae. J Gen Virol 70 : 2811–2817. [Google Scholar]
  32. Siebler J, Haller O, Kocks G, 1996. Thogoto and Dhori virus replication is blocked by inhibitiors of cellular polymerase II activity but does not cause shutoff of host cell protein synthesis. Arch Virol 141 : 1587–1594. [Google Scholar]
  33. Leahy MB, Dessens JT, Weber F, Kocks G, Nuttall PA, 1997. The fourth genus in the Orthomyxoviridae: sequence analyses of two Thogoto virus polymerase proteins and comparison with Influenza viruses. Virus Res 50 : 215–224. [Google Scholar]
  34. Lamb RA, Krug RM, 2001. Orthomyxoviridae: The Viruses and Their Replication. Knipe DM, Howley PM, eds. Fields Virology. Fourth edition. Philadelphia: Lippincott Williams and Wilkins, 1487–1532.

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  • Received : 20 May 2005
  • Accepted : 19 Aug 2005

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