1921
Volume 73, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

A cross-sectional study of infection in children who lived in an orphanage in Bangkok, Thailand was conducted in April 2003. Two hundred ninety stool specimens were collected and examined under light microscopy after staining with gram-chromotrope. Confirmation of was done using transmission electron microscopy. Of 290 samples, 12 (4.1%) were positive for . Genotypic characterization of 10 showed that all were genotype A, which might indicate the same source of infection. Multivariate analysis showed that orphans who were 12–23 months old, girls, and living in one particular house were independently associated with infection. Our study suggests that infection in this orphanage might be transmitted person to person.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.2005.73.104
2005-07-01
2018-12-11
Loading full text...

Full text loading...

/deliver/fulltext/14761645/73/1/0730104.html?itemId=/content/journals/10.4269/ajtmh.2005.73.104&mimeType=html&fmt=ahah

References

  1. Desportes I, Le Charpentier Y, Galian A, Bernard F, Cochand-Priollet B, Lavergne A, Ravisse P, Modigliani R, 1985. Occurrence of a new microsporidian: Enterocytozoon bieneusi n.g., n. sp., in the enterocytes of a human patient with AIDS. J Protozool 32 : 250–254. [Google Scholar]
  2. Cali A, Kotler DP, Orenstein JM, 1993. Septata intestinalis N.G., N. Sp., an intestinal microsporidian associated with chronic diarrhea and dissemination in AIDS patients. J Eukaryot Microbiol 40 : 101–112. [Google Scholar]
  3. Keeling PJ, Fast NM, 2002. Microsporidia: biology and evolution of highly reduced intracellular parasites. Annu Rev Microbiol 56 : 93–116. [Google Scholar]
  4. Kotler DP, Orenstein JM, 1998. Clinical syndromes associated with microsporidiosis. Adv Parasitol 40 : 321–349. [Google Scholar]
  5. Franzen C, Müller A, 2001. Microsporidiosis: human diseases and diagnosis. Microbes Infect 3 : 389–400. [Google Scholar]
  6. Gambo T, Hobbs RE, Carlyn C, Hall G, Isada CM, 1999. Microsporidia infection in transplant patients. Transplantation 67 : 482–484. [Google Scholar]
  7. Sandfort J, Hannemann A, Gelderblom H, Stark K, Owen RL, Ruf B, 1994. Enterocytozoon bieneusi infection in an immunocompetent patient who had acute diarrhea and who was not infected with the human immunodeficiency virus. Clin Infect Dis 19 : 514–516. [Google Scholar]
  8. Sobottka I, Albrecht H, Schottelius J, Schmetz C, Bentfeld M, Laufs R, Schwartz DA, 1995. Self-limited traveller’s diarrhea due to a dual infection with Enterocytozoon bieneusi and Cryptosporidium parvum in an immunocompetent HIV-negative child. Eur J Clin Microbiol Infect Dis 14 : 919–920. [Google Scholar]
  9. Hutin YJF, Sombardier M-N, Liguory O, Sarfati C, Derouin F, Modai J, Molina JM, 1998. Risk factors for intestinal microsporidiosis in patients with human immunodeficiency virus infection: a case-control study. J Infect Dis 178 : 904–907. [Google Scholar]
  10. Gumbo T, Sarbah S, Gangaidzo IT, Ortega Y, Sterling CR, Carville A, Tzipori S, Wiest PM, 1999. Intestinal parasites in patients with diarrhea and human immunodeficiency virus infection in Zimbabwe. AIDS 13 : 819–821. [Google Scholar]
  11. Mungthin M, Suwannasaeng R, Naaglor T, Areekul W, Leelayoova S, 2001. Asymptomatic intestinal microsporidiosis in Thai orphans and child-care workers. Trans R Soc Trop Med Hyg 95 : 304–306. [Google Scholar]
  12. Cegielski JP, Ortega YR, McKee S, Madden JF, Gaido L, Schwartz DA, Manji K, Jorgensen AF, Miller SE, Pulipaka UP, Msengi AE, Mwakyusa DH, Sterling CR, Reller LB, 1999. Cryptosporidium, Enterocytozoon, and Cyclospora infections in pediatric and adult patients with diarrhea in Tanzania. Clin Infect Dis 28 : 314–321. [Google Scholar]
  13. Moura H, da Silva JL, Sodre FC, Brasil P, Walmo D, Wahlquist S, Wallace S, Croppo GP, Visvesvara GS, 1996. Gram-chromotrope: a new technique that enhances detection of microsporidial spores in clinical samples. J Eukaryot Microbiol 43 : 94S–95S. [Google Scholar]
  14. Visvesvara GS, Leitch GJ, Moura H, Wallace S, Weber R, Bryan RT, 1991. Culture, electron microscopy, and immunoblot studies on a microsporidian parasite isolated from the urine of a patient with AIDS. J Protozool 38 : 105S–111S. [Google Scholar]
  15. Katzwinkel-Wladarsch S, Lieb M, Heise W, Löscher T, Rinder H, 1996. Direct amplification and species determination of microsporidian DNA from stool specimens. Trop Med Int Health 1 : 373–378. [Google Scholar]
  16. Leelayoova S, Vithayasai N, Watanaveeradej V, Chotpitayasunondh T, Therapong V, Naaglor T, Mungthin M, 2001. Intestinal microsporidiosis in HIV-infected children with acute and chronic diarrhea. Southeast Asian J Trop Med Public Health 32 : 33–37. [Google Scholar]
  17. Wanachiwanawin D, Chokephaibulkit K, Lertlaituan P, Ongrotchanakun J, Chinabut P, Thakerngpol K, 2002. Intestinal microsporidiosis in HIV-infected children with diarrhea. Southeast Asian J Trop Med Public Health 33 : 241–245. [Google Scholar]
  18. Tumwine JK, Kekitiinwa A, Nabukeera N, Akiyoshi DE, Buckholt MA, Tzipori S, 2002. Enterocytozoon bieneusi among children with diarrhea attending Mulago hospital in Uganda. Am J Trop Med Hyg 67 : 299–303. [Google Scholar]
  19. Dengjel B, Zahler M, Hermanns W, Heinritzi K, Spillmann T, Thomschke A, Loscher T, Gothe R, Rinder H, 2001. Zoonotic potential of Enterocytozoon bieneusi. J Clin Microbiol 39 : 4495–4499. [Google Scholar]
  20. Sadler F, Peake N, Borrow R, Rowl PL, Wilkins EG, Curry A, 2002. Genotyping of Enterocytozoon bieneusi in AIDS patients from the north west of England. J Infect 44 : 39–42. [Google Scholar]
  21. Sulaiman IM, Bern C, Gilman R, Cama V, Kawai V, Vargas D, Ticona E, Vivar A, Xiao L, 2003. A molecular biological study of Enterocytozoon bieneusi in HIV-infected patients in Lima, Peru. J Eukaryot Microbiol 50 (suppl): 591–596. [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2005.73.104
Loading
/content/journals/10.4269/ajtmh.2005.73.104
Loading

Data & Media loading...

  • Received : 11 Oct 2004
  • Accepted : 25 Nov 2004

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error