Volume 72, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Cathepsin L1, a cysteine protease secreted by the gastrodermis of juvenile and adult , was expressed in as a calmodulin binding peptide fusion protein with a molecular mass of approximately 35 kD. The recombinant cathepsin L1 (rCTL1) was tested for its antigenic potential in a cystatin capture enzyme-linked immunosorbent assay (ELISA) to diagnose human fascioliasis. The ELISA plates were sensitized with chicken egg cystatin and incubated with bacterial lysates containing the recombinant protein before the standard ELISA procedures were performed. Analysis of the sera of 13 patients infected with (group 1), 204 patients with other parasitic infections (group 2), 32 cholangiocarcinoma patients (group 3), and 42 healthy controls (group 4) showed that the sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of this ELISA using rCTL1 were 100%, 98.92%, 98.97%, 81.25%, and 100%, respectively. These results indicate that this assay has high sensitivity and specificity in the diagnosis of human fascioliasis. In addition, we have produced sufficient amounts of antigen for use in diagnosis.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...



  1. World Health Organization, 1995. Control of foodborne trematode infections. World Health Organ Tech Rep Ser 849 : 1–157. [Google Scholar]
  2. Mas-Coma S, Bargues MD, Esteban JG, 1998. Human fasciolosis. Dalton JP, ed. Fasciolosis. Wallingford, United Kingdom: CABI Publishing, 411–434.
  3. Andrews SJ, 1998. The life cycle of Fasciola hepatica. Dalton JP, ed. Fasciolosis. Wallingford, United Kingdom: CABI Publishing, 1–29.
  4. Maleewong W, Wongkham C, Intapan PM, Pipitgool V, 1999. Fasciola gigantica-specific antigens: purification by a continuous-elution method and its evaluation for the diagnosis of human fascioliasis. Am J Trop Med Hyg 61 : 648–651. [Google Scholar]
  5. Grams R, Vichasri-Grams S, Sobhon P, Upatham ES, Viyanant V, 2001. Molecular cloning and characterization of cathepsin L encoding genes from Fasciola gigantica. Parasitol Int 50 : 105–114. [Google Scholar]
  6. O’Neill SM, Parkinson M, Strauss W, Angles R, Dalton JP, 1998. Immunodiagnosis of Fasciola hepatica infection (fascioliasis) in a human population in the Bolivian Altiplano using purified cathepsin L cysteine proteinase. Am J Trop Med Hyg 58 : 417–423. [Google Scholar]
  7. O’Neill SM, Parkinson M, Dowd AJ, Strauss W, Angles R, Dalton JP, 1999. Immunodiagnosis of human fascioliasis using recombinant Fasciola hepatica cathepsin L1 cysteine proteinase. Am J Trop Med Hyg 60 : 749–751. [Google Scholar]
  8. Strauss W, O’Neill SM, Parkinson M, Angles R, Dalton JP, 1999. Diagnosis of human fascioliasis: detection of anti-cathepsin L antibodies in blood samples collected on filter paper. Am J Trop Med Hyg 60 : 746–748. [Google Scholar]
  9. Carnevale S, Rodriguez MI, Guarnera EA, Carmona C, Tanos T, Angel SO, 2001. Immunodiagnosis of fasciolosis using recombinant procathepsin L cystein proteinase. Diagn Microbiol Infect Dis 41 : 43–49. [Google Scholar]
  10. Cornelissen JB, Gaasenbeek CP, Borgsteede FH, Holland WG, Harmsen MM, Boersma WJ, 2001. Early immunodiagnosis of fasciolosis in ruminants using recombinant Fasciola hepatica cathepsin L-like protease. Int J Parasitol 31 : 728–737. [Google Scholar]
  11. Rokni MB, Massoud J, O’Neill SM, Parkinson M, Dalton JP, 2002. Diagnosis of human fasciolosis in the Gilan province of northern Iran: application of cathepsin L-ELISA. Diagn Microbiol Infect Dis 44 : 175–179. [Google Scholar]
  12. Erdman DD, 1981. Clinical comparison of ethyl acetate and di-ethyl ether in the formalin-ether sedimentation technique. J Clin Microbiol 14 : 483–485. [Google Scholar]
  13. Chomczynski P, Sacchi N, 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162 : 156–159. [Google Scholar]
  14. Cohen SN, Chang AC, 1977. Revised interpretation of the origin of the pSC101 plasmid. J Bacteriol 132 : 734–737. [Google Scholar]
  15. Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 : 248–254. [Google Scholar]
  16. Laemmli UK, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 : 680–685. [Google Scholar]
  17. Towbin H, Staehelin T, Gordon J, 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 76 : 4350–4354. [Google Scholar]
  18. Ikeda T, 1998. Cystatin capture enzyme-linked immunosorbent assay for immunodiagnosis of human paragonimiasis and fascioliasis. Am J Trop Med Hyg 59 : 286–290. [Google Scholar]
  19. Galen RS, 1980. Predictive value and efficiency of laboratory testing. Pediatr Clin North Am 27 : 861–869. [Google Scholar]
  20. Anastasi A, Brown MA, Kembhavi AA, Nicklin MJ, Sayers CA, Sunter DC, Barrett AJ, 1983. Cystatin, a protein inhibitor of cysteine proteinases. Improved purification from egg white, characterization, and detection in chicken serum. Biochem J 211 : 129–138. [Google Scholar]

Data & Media loading...

  • Received : 01 Apr 2004
  • Accepted : 30 Apr 2004

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error