1921
Volume 72, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Volunteers vaccinated with a candidate malaria vaccine containing merozoite surface protein 1 (MSP-1) exhibit antibodies to MSP-1 that are measured by enzyme-linked immunosorbent assay (ELISA). The purpose of this study was to make a human reference standard for MSP-1 antibody measured in absolute quantity units using pooled plasma samples known to contain high titers of MSP-1 antibody based on previous ELISA results. Immobilized metal affinity chromatography was used to determine the amount of MSP-1 antibody in this plasma pool. Hexahistidine-tagged MSP-1 antigen adsorbed to nickel-chelating resin was used to capture MSP-1 antibody from the plasma pool. The intact MSP-1 antibody-antigen complexes were eluted and total IgG was measured by an ELISA standardized against purified human IgG. In this way, the human reference standard was determined to contain 48.3 μg/mL of MSP-1 antibody. This reference standard may be useful as a quantitative working standard for measuring MSP-1 antibody response in future vaccine clinical trials involving MSP-1.

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  • Received : 11 Aug 2004
  • Accepted : 07 Oct 2004

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