Volume 72, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


infection causes scrub typhus, a common zoonosis of rural Asia. was recently detected by a real-time quantitative polymerase chain reaction (qPCR) assay in animal specimens. We evaluated the same qPCR assay in specimens obtained from patients with serologically proven scrub typhus infections. The 47-kDa qPCR assay was more sensitive than was mouse inoculation; it was reactive in whole blood specimens from all 10 isolate-positive patients and in 7 of 17 isolate-negative individuals ( = 0.003, Fisher’s two-tailed exact test). As few as 1,076 copies/μL were detected in whole blood. Four of 7 sera from isolate-proven scrub typhus infections were also reactive by qPCR. The assay was unreactive in all 12 individuals without scrub typhus infection. This is the first demonstration of a sensitive and specific real-time qPCR assay for human scrub typhus infection.


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  • Received : 26 May 2004
  • Accepted : 30 Aug 2004

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