1921
Volume 72, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

An estimated 12 million persons throughout the world suffer from the protozoan disease leishmaniasis. Current treatments have liabilities including poor activity against some forms of leishmaniasis, toxicity, or the need for parenteral administration. Higher throughput methods to screen chemical compounds are needed to facilitate the search for new antileishmania drugs. In the mammalian host, parasites exist as amastigotes that replicate within macrophages. Therefore, an screening assay using intramacrophage amastigotes most closely represents the natural infection. We have transfected strains of and with the β-lactamase gene, which catalyzes a colorimetric reaction with the substrate nitrocephin. The growth of these β-lactamase–expressing within macrophages was quantified in 96-well plates using an optical density plate reader, thus simplifying the methodology for scoring inhibitor assays. This simple and relatively inexpensive colorimetric assay helps improve throughput for screening compounds for antileishmania activity.

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2005-05-01
2017-09-21
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  • Received : 24 Jun 2004
  • Accepted : 11 Nov 2004

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