Volume 72, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Samples of laboratory propagated Venezuelan equine encephalitis (VEE), West Nile (WN), and yellow fever (YF) viruses were blotted onto filter paper discs, air-dried, and stored at room temperature. At regular intervals over a 90-day period, the dried virus samples were eluted, tested for infectivity by culture and titration in Vero cells, and examined for viral RNA by a reverse transcriptase–polymerase chain reaction. The VEE, WN, and YF viral RNA was detected throughout the 90-day period in all samples examined. Infectious VEE virus could be recovered for up to 40 days; WN and YF viruses were cultured in Vero cells for up to 60 and 90 days, respectively. The results of this study demonstrate that viral nucleic acids and infectious virus can be recovered from arbovirus samples air-dried on filter paper and stored at room temperature for a month or more after collection. This procedure offers a simple and inexpensive method for collecting arbovirus field specimens and transporting them to diagnostic laboratories.


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  1. Wacharapluesadee S, Phumesin P, Lumlertdaecha B, Hemachudha T, 2003. Diagnosis of rabies by use of brain tissue dried on filter paper. Clin Infect Dis 36 : 674–675.
    [Google Scholar]
  2. Beck I, Brennan KD, Melvin AJ, Mohan KM, Herz AM, Alarcon J, Piscoya J, Valesquez C, Frenkel LM, 2001. Simple, sensitive and specific detection of human immunodeficiency virus type 1 subtype B DNA in dried blood samples for diagnosis in infants in the field. J Clin Microbiol 39 : 29–33.
    [Google Scholar]
  3. Fischer A, Lejezak C, Lambert C, Servais J, Makombe N, Rusine J, Staub T, Hemmer R, Schneider F, Schmit JC, Arendt V, 2004. Simple DNA extraction method for dried blood spots and comparison of two PCR assays for diagnosis of vertical human immunodeficiency virus type 1 transmission in Rwanda. J Clin Microbiol 42 : 16–20.
    [Google Scholar]
  4. Tonry J, Xiao SY, Siirin M, Chen H, Travassos da Rosa APA, Tesh RB, 2005. Persistent shedding of West Nile virus in urine of experimentally infected hamsters. Am J Trop Med Hyg 72 : 320–324.
    [Google Scholar]
  5. Tesh RB, Guzman H, Travassos da Rosa APA, Vasconcelos PFC, Dias LB, Bunnel JE, Zhang H, Xiao SY, 2001. Experimental yellow fever virus infection in the golden hamster (Mesocricetus auratus). 1. Virologic, biochemical and immunologic studies. J Infect Dis 183 : 1431–1436.
    [Google Scholar]
  6. Walder R, Liprandi F, 1976. Kinetics of inactivation of Venezuelan equine encephalomyelitis virus. Arch Virol 51 : 307–317.
    [Google Scholar]
  7. Fleming P, 1971. Thermal inactivation of Semliki Forest virus. J Gen Virol 13 : 385–391.
    [Google Scholar]
  8. Sithisarn P, Suksanpaisan L, Thepparit C, Smith DR, 2003. Behavior of dengue virus in solution. J Med Virol 71 : 532–539.
    [Google Scholar]
  9. Mayo DR, Beckwith WH, 2002. Inactivation of West Nile virus during serologic testing and transport. J Clin Microbiol 40 : 3044–3046.
    [Google Scholar]
  10. Pikal MJ, 2004. Mechanisms of protein stabilization during freeze-drying and storage; the relative importance of thermodynamic stabilization and glassy state relaxation dynamics. Rey L, May JC, eds. Freeze-Drying of Pharmaceutical and Biological Products. Second edition. New York: Marcel Dekker Inc., 63–107.

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  • Received : 09 Jun 2004
  • Accepted : 09 Oct 2004
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