Volume 72, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


We evaluated the use of 7SL RNA gene sequences for the identification of spp. A fragment (~137 basepairs) of the 7SL RNA gene from 13 reference strains and 18 clinical isolates of 11 different species was amplified and sequenced using conserved primers. Reference strains from each spp. complex showed unique sequences. The nucleotide sequences were compared pairwise and a range of 81.0–99.3% intercomplex similarity was observed. Clinical isolates of the same species had sequences identical to the corresponding reference strains; thus, the intraspecies similarity was 100%. A phylogenetic tree derived from the 7SL RNA gene partial sequences was constructed and is in agreement with accepted phylogenetic schemes.


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  • Received : 26 Aug 2004
  • Accepted : 24 Nov 2004

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