1921
Volume 72, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The binding of dengue type 2 (DEN-2) virus to mammalian (LLC-MK and Vero) and mosquito (C6/36 and AP61) cell surfaces was investigated by a virus-binding assay using purified H-labeled DEN-2 virus. The DEN-2 virus binding to all four cell types was specific and saturable, indicating the presence of a single class of receptors (ranging from 3.7 × 10 to 3.5 × 10 receptors/cell) with a high affinity for DEN-2 virus ( ranging from 98 to 171 pM). Treatment of cell surfaces with certain glycosidases significantly reduced virus binding to mammalian cell lines, but not to the insect cell lines examined. Furthermore, heparin was found to compete with mammalian cell receptors for binding to DEN-2 virus and to inhibit viral infection of mammalian cells, but heparin had no effect on viral binding to or infection of insect cells. These results confirm previous reports suggesting that DEN-2 virus receptors on mammalian cell lines are different from those on insect cell lines.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2005.72.375
2005-04-01
2017-09-23
Loading full text...

Full text loading...

/deliver/fulltext/14761645/72/4/0720375.html?itemId=/content/journals/10.4269/ajtmh.2005.72.375&mimeType=html&fmt=ahah

References

  1. Gubler DJ, 1997. Dengue and dengue hemorrhagic fever: its history and resurgence as a global public health problem. Gubler DJ, Kuno G, eds. Dengue and Dengue Hemorrhagic Fever. New York: CAB International, 1–22.
  2. Innis BL, 1995. Dengue and dengue hemorrhagic fever. Porterfield JS, ed. Kass Handbook of Infectious Diseases: Exotic Viral Infections. London: Chapman and Hall: 103–146.
  3. Chambers TJ, Hahn CS, Galler R, Rice CM, 1990. Flavivirus genome organization, expression, and replication. Annu Rev Microbiol 44 : 649–688.
  4. Rice CM, Lenches EM, Eddy SR, Shin SJ, Sheets RL, Strauss JH, 1985. Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science 229 : 726–733.
  5. Westaway EG, Brinton MA, Gaidamovich SYa, Horzinek MC, Igarashi A, Kaariainen L, Lvov DK, Porterfield JS, Russell PK, Trent DW, 1985. Flaviviridae. Intervirology 24 : 183–192.
  6. Anderson R, King AD, Innis BL, 1992. Correlation of E protein binding with cell susceptibility to dengue 4 virus infection. J Gen Virol 73 : 2155–2159.
  7. Chen Y, Maguire T, Marks RM, 1996. Demonstration of binding of dengue virus envelope protein to target cells. J Virol 70 : 8765–8772.
  8. He R, Innis BL, Nisalak A, Usawattanakul W, Wang S, Kalaya-narooj S, Anderson R, 1995. Antibodies that block virus attachment to Vero cells are a major component of the human neutralizing antibody response against dengue virus type 2. J Med Virol 45 : 451–461.
  9. Holzman H, Stiasny K, York H, Dorner F, Kunz C, Heinz FX, 1995. Tick-borne encephalitis virus envelope protein E-specific monoclonal antibodies for the study of low pH-induced conformational changes and immature virions. Arch Virol 140 : 213–221.
  10. Wang S, He R, Patarapotikul J, Innis BL, Anderson R, 1995. Antibody-enhanced binding of dengue-2 virus to human platelets. Virology 213 : 254–257.
  11. Gollins SW, Porterfield JS, 1985. Flavivirus infection enhancement in macrophages: an electron microscopic study of viral cellular entry. J Gen Virol 66 : 1969–1982.
  12. Chen Y, Maguire T, Hileman RE, Fromm JR, Esko JD, Linhardt RJ, Marks RM, 1997. Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate. Nature Med 3 : 866–871.
  13. Hase T, Summers PL, Eckels KH, 1989. Flavivirus entry into cultured mosquito cells and human peripheral blood monocytes. Arch Virol 104 : 129–143.
  14. Daughaday CC, Brandt WE, McCown JM, Russell PK, 1981. Evidence for two mechanisms of dengue virus infection of adherent human monocytes: trypsin-sensitive virus receptors and trypsin-resistant immune complex receptors. Infect Immun 32 : 469–473.
  15. Rothwell SW, Putnak R, La Russa VF, 1996. Dengue-2 virus infection of human bone marrow: characterization of dengue-2 antigen positive stromal cells. Am J Trop Med Hyg 54 : 503–510.
  16. Ramos-Castaneda J, Imbert JL, Barron BL, Ramos C, 1997. A 65-kDa trypsin-sensible membrane cell protein as a possible receptor for dengue virus in cultured neuroblastoma cells. J Neurovirol 3 : 435–440.
  17. Munoz ML, Cisneros A, Cruz J, Das P, Tovar R, Ortega A, 1998. Putative dengue virus receptors from mosquito cells. FEMS Microbiol Lett 168 : 251–258.
  18. Moreno-Altamirano MMB, Sanchez-Garcia FJ, Munoz ML, 2002. Non Fc receptor-mediated infection of human macrophages by dengue virus serotype 2. J Gen Virol 83 : 1123–1130.
  19. Hung SL, Lee PL, Chen HW, Chen LK, Kao CL, King CC, 1999. Analysis of the steps involved in dengue virus entry into host cells. Virology 257 : 156–167.
  20. Rostand KS, Esko JD, 1997. Microbial adherence to and invasion through proteoglycans. Infect Immun 65 : 1–8.
  21. Varma MGR, Pudney M, Leake CJ, 1974. Cell lines from larvae of Aedes (Stegomyia) malayensis (Colless) and Aedes (S.) pseudoscutellaris (Theobald) and their infection with some arboviruses. Trans R Soc Trop Med Hyg 68 : 374–382.
  22. Russell PK, Nisalak A, 1967. Dengue virus identification by the plaque reduction neutralization test. J Immunol 99 : 291–296.
  23. Russell PK, Nisalak A, Sukhavachana P, Vivova S, 1967. A plaque reduction test for dengue virus neutralizing antibodies. J Immunol 99 : 285–290.
  24. Clayson ET, Compans RW, 1988. Entry of simian virus 40 is restricted to apical surfaces of polarized epithelial cells. Mol Cell Biol 8 : 3391–3396.
  25. Henchal EA, Putnak JR, 1990. The dengue viruses. Clin Microbiol Rev 3 : 376–396.
  26. Clayson ET, Compans RW, 1989. Characterization of simian virus 40 receptor moieties on the surfaces of Vero C1008 cells. J Virol 63 : 1095–1100.
  27. Henchal EA, Gentry MK, McCown JM, Brandt WE, 1982. Dengue virus-specific and flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am J Trop Med Hyg 31 : 830–836.
  28. Scatchard G, 1949. The attraction of proteins for small molecules and ions. Ann N Y Acad Sci 51 : 660–672.
  29. Hill AV, 1913. The combination of hemoglobin with oxygen and carbon monoxide. Biochem J 7 : 471–480.
  30. Kalyanaraman VS, Sarngadharan MG, Gallo RC, 1978. Characterization of Rauscher murine leukemia virus envelope glycoprotein receptor in membranes from murine fibroblasts. J Virol 28 : 686–696.
  31. Fries E, Helenius A, 1979. Binding of Semliki Forest virus and its spike glycoproteins to cells. Eur J Biochem 97 : 213–220.
  32. Schlegel R, Willingham MC, Pastan IH, 1982. Saturable binding sites for vesicular stomatitis virus on the surface of Vero cells. J Virol 43 : 871–875.
  33. Epstein RL, Powers ML, Rogart RB, Weiner HL, 1984. Binding of 125I-labeled reovirus to cell surface receptors. Virology 133 : 46–55.
  34. Persson R, Wohlfart C, Svensson V, Everitt E, 1985. Virus-receptor interaction in the adenovirus system: characterization of the positive cooperative binding of virions on HeLa cells. J Virol 54 : 92–97.
  35. Johnson PA, Rosner MR, 1986. Characterization of murine-specific leukemia virus receptor from L cells. J Virol 58 : 900–908.
  36. Peeples ME, Komai K, Radek R, Bankowski MJ, 1987. A cultured cell receptor for the small S protein of hepatitis B virus. Virology 160 : 135–142.
  37. Salas-Benito JS, del Angel RM, 1997. Identification of two surface proteins from C6/36 cells that bind dengue type 4 virus. J Virol 71 : 7246–7252.
  38. Stollar V, 1980. Togaviruses in cultured arthropod cells. Schle-singer RW, ed. The Togaviruses: Biology, Structure, Replication. New York: Academic Press, 584–621.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2005.72.375
Loading
/content/journals/10.4269/ajtmh.2005.72.375
Loading

Data & Media loading...

  • Received : 29 Aug 2003
  • Accepted : 25 Sep 2003

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error