Volume 72, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Recent findings indicating a low level of polymorphism in the genome have led to the hypothesis that existent polymorphisms are likely to have functional significance. We tested this hypothesis by developing a map of the polymorphism in the multidrug resistance 1 () gene 5′ upstream region and assaying its correlation with drug resistance in a sample of field isolates from Dakar, Senegal. A comparison of six geographically diverse laboratory strains showed that the 1.94-kb 5′-untranslated region is highly monomorphic, with a total of four unique single nucleotide polymorphisms (SNPs) being identified. All of the mutations were localized to a 462-basepair region proximal to the transcription start point. Analysis of this region in field isolates shows the prevalence of one SNP throughout the entire population of parasites, irrespective of drug resistance status. The SNP frequency of the upstream region is lower than that found in the noncoding region of other genes.


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  • Received : 28 Jul 2003
  • Accepted : 07 Jul 2004

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