Volume 71, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


To study the genetic diversity of re-emerging in the Republic of Korea, nucleotide sequence variations at the merozoite surface protein-3α (PvMSP-3α) locus were analyzed using 24 re-emerging isolates and 4 isolates from imported cases. Compared with the well known Belem strain (Brazil), a large number of amino acid substitutions, deletions, and insertions were found at the locus of the isolates examined. The Korean isolates were divided into two allelic types; type I (15 isolates), similar to the Belem strain, and type II (9), similar to the Chess strain (New Guinea). Isolates from imported cases were classified into three types; type III (1 from Malaysia), similar to type B from western Thailand, type IV (1 each from Indonesia and India), and type V (1 from Pakistan), both being new types. Our results have shown that the MSP-3α locus of re-emerging Korean is dimorphic with two allelic types coexisting in the endemic area.


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  1. World Health Organization, 1997. World malaria situation in 1994–Part I. Wkly Epidemiol Rec 72 : 269–274. [Google Scholar]
  2. Mendis K, Sina BJ, Marchesini P, Carter R, 2001. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 64 (Suppl): 97–106. [Google Scholar]
  3. Chai IH, Lim GI, Yoon SN, Oh WI, Kim SJ, Chai JY, 1994. Occurrence of tertian malaria in a male patient who has never been abroad. Korean J Parasitol 32 : 195–200. [Google Scholar]
  4. Chai JY, 1999. Re-emerging Plasmodium vivax malaria in the Republic of Korea. Korean J Parasitol 37 : 129–143. [Google Scholar]
  5. Park JW, Klein TA, Lee HC, Pacha LA, Ryu SH, Yeom JS, Moon SH, Kim TS, Chai JY, Oh MD, Choe KW, 2003. Vivax malaria: a continuing health threat to the Republic of Korea. Am J Trop Med Hyg 69 : 159–167. [Google Scholar]
  6. Center for Disease Control and Prevention, Korea, 2003. Statistics. Communicable Diseases Monthly Report, Korea 15: 10–17 (in Korean).
  7. Kumar S, Epstein JE, Richie TL, 2002. Vaccines against asexual stage malaria parasites. Chem Immunol 80 : 262–286. [Google Scholar]
  8. Mahanty S, Saul A, Miller LH, 2003. Progress in the development of recombinant and synthetic blood-stage malaria vaccines. J Exp Biol 206 : 3781–3788. [Google Scholar]
  9. Oeuvray C, Bouharoun-Tayoun H, Gras-Masse H, Bottius E, Kaidoh T, Aikawa M, Filgueira MC, Tartar A, Druilhe P, 1994. Merozoite surface protein-3: a malaria protein inducing antibodies that promote Plasmodium falciparum killing by cooperation with blood monocytes. Blood 84 : 1594–1602. [Google Scholar]
  10. Hisaeda H, Saul A, Reece JJ, Kennedy MC, Long CA, Miller LH, Stowers AW, 2002. Merozoite surface protein-3 and protection against malaria in Aotus nancymai monkeys. J Infect Dis 185 : 657–664. [Google Scholar]
  11. Galinski MR, Corredor-Medina C, Povoa M, Crosby J, Ingravallo P, Barnwell JW, 1999. Plasmodium vivax merozoite surface protein-3 contains coiled-coil motifs in an alanine-rich central domain. Mol Biochem Parasitol 101 : 131–147. [Google Scholar]
  12. Galinski MR, Ingravallo P, Corredor-Medina C, Al-Khedery B, Povoa M, Barnwell JW, 2001. Plasmodium vivax merozoite surface proteins-3β and −3γ share structural similarities with P. vivax merozoite surface protein-3α and define a new gene family. Mol Biochem Parasitol 115 : 41–53. [Google Scholar]
  13. Rayner JC, Corredor V, Feldman D, Ingravallo P, Iderabdullah F, Galinski MR, Barnwell JW, 2002. Extensive polymorphism in the Plasmdium vivax merozoite surface coat protein MSP-3α is limited to specific domains. Parasitology 125 : 393–405. [Google Scholar]
  14. McColl DJ, Silva A, Foley M, Kun JFJ, Favaloro JM, Thompson JK, Marshall VM, Coppel RL, Kemp DJ, Anders RF, 1994. Molecular variation in a novel polymorphic antigen associated with Plasmodium falciparum merozoites. Mol Biochem Parasitol 68 : 53–67. [Google Scholar]
  15. Badell E, Oeuvray C, Moreno A, Soe S, van Rooijen N, Bouzidi A, Druilhe P, 2001. Human malaria in immunocompromised mice: an in vivo model to study defense mechanisms against Plasmodium falciparum. J Exp Med 192 : 1653–1659. [Google Scholar]
  16. Bruce MC, Galinski MR, Barnwell JW, Snounou G, Day KP, 1999. Polymorphism at the merozoite surface protein-3α locus of Plasmodium vivax: global and local diversity. Am J Trop Med Hyg 61 : 518–525. [Google Scholar]
  17. Bruce MC, Galinski MR, Barnwell JW, Donnelly CA, Walmsley M, Alpers MP, Walliker D, Day KP, 2000. Genetic diversity and dynamics of Plasmodium falciparum and P. vivax populations in multiply infected children with asymptomatic malaria infections in Papua New Guinea. Parasitology 121 : 257–272. [Google Scholar]
  18. Cui L, Mascorro CN, Fan Q, Rzomp KA, Khuntirat B, Zhou G, Chen H, Yan G, Sattabongkot J, 2003. Genetic diversity and multiple infections of Plasmodium vivax malaria in western Thailand. Am J Trop Med Hyg 68 : 613–619. [Google Scholar]
  19. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG, 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 : 4876–4882. [Google Scholar]
  20. Kho WG, Park YH, Chung JY, Kim JP, Hong ST, Lee WJ, Kim TS, Lee JS, 1999. Two new genotypes of Plasmodium vivax circumsporozoite protein found in the Republic of Korea. Korean J Parasitol 37 : 265–270. [Google Scholar]
  21. Lim CS, Kim YK, Lee KN, Kim SH, Hoffman KJ, Song KJ, Song JW, 2001. The analysis of circumsporozoite-protein gene sequences from South Korean isolates of Plasmodium vivax. Ann Trop Med Parasitol 95 : 229–235. [Google Scholar]
  22. Kho WG, Chung JY, Sim EJ, Kim DW, Chung WC, 2001. Analysis of polymorphic regions of Plasmodium vivax Duffy binding protein of Korean isolates. Korean J Parasitol 39 : 143–150. [Google Scholar]
  23. Han ET, Park JH, Shin EH, Choi MH, Oh MD, Chai JY, 2002. Apical membrane antigen-1 (AMA-1) gene sequences of re-emerging Plasmodium vivax in South Korea. Korean J Parasitol 40 : 157–162. [Google Scholar]
  24. Chung JY, Chun EH, Chun JH, Kho WG, 2003. Analysis of the Plasmodium vivax apical membrane antigen-1 gene from re-emerging Korean isolates. Parasitol Res 90 : 325–329. [Google Scholar]
  25. Lim CS, Kim SH, Kwon SI, Song JW, Song KJ, Lee KN, 2000. Analysis of Plasmodium vivax merozoite surface protein-1 gene sequences from resurgent Korean isolates. Am J Trop Med Hyg 62 : 261–265. [Google Scholar]
  26. Putaporntip C, Jongwutiwes S, Sakihama N, Ferreira MU, Kho WG, Kaneko A, Kanbara H, Hattori T, Tanabe K, 2002. Mosaic organization and heterogeneity in frequency of allelic recombination of the Plasmodium vivax merozoite surface protein-1 locus. Proc Natl Acad Sci USA 99 : 16348–16353. [Google Scholar]
  27. Chai JY, Park YK, Guk SM, Oh KH, Oh MD, Lee SH, Kim HS, Wataya Y, 2000. A trial for a DNA diagnosis of Plasmodium vivax malaria recently reemerging in the Republic of Korea using microtiter plate hybridization assay. Am J Trop Med Hyg 63 : 80–84. [Google Scholar]

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  • Received : 09 Mar 2004
  • Accepted : 02 Jul 2004

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