1921
Volume 71, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

With the implementation of programs to control lymphatic filariasis and soil-transmitted helminths using broad spectrum anthelmintics, including albendazole and ivermectin, there is a need to develop an assay for detection of drug resistance. This report describes an assay for measuring the effects of ivermectin and benzimidazoles on the motility of larvae of the hookworm species , and and species including , and . A dose-response relationship was demonstrated with each of the parasite species, with distinct differences observed between the various species. In pilot field testing of the assay with larvae recovered from human fecal samples, a dose-response relationship was observed with ivermectin. While the assay has demonstrated the ability to determine drug responsiveness, its usefulness in resistance detection will require correlation with the clinical outcome among individuals infected with parasite strains showing different drug sensitivities.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2004.71.608
2004-11-01
2017-09-26
Loading full text...

Full text loading...

/deliver/fulltext/14761645/71/5/0700608.html?itemId=/content/journals/10.4269/ajtmh.2004.71.608&mimeType=html&fmt=ahah

References

  1. Ottesen EA, 2000. The global programme to eliminate lymphatic filariasis. Trop Med Int Health 5 : 591–594.
  2. Simeon DT, Grantham-McGregor SM, Callender JE, Wong MS, 1995. Treatment of Trichuris trichiura infections improves growth, spelling scores and school attendance in some children. J Nutr 125 : 1875–1883.
  3. Nokes C, Grantham-McGregor SM, Sawyer AW, Cooper ES, Robinson BA, Bundy DA, 1992. Moderate to heavy infections of Trichuris trichiura affect cognitive function in Jamaican school children. Parasitology 104 : 539–547.
  4. Winnen M, Plaisier AP, Alley ES, Nagelkerke NJ, van Oortmarssen G, Boatin BA, Habbema JD, 2002. Can ivermectin mass treatments eliminate onchocerciasis in Africa? Bull World Health Organ 80 : 384–391.
  5. Prichard R, 1994. Anthelmintic resistance. Vet Parasitol 54 : 259–268.
  6. Sangster NC, 1999. Anthelmintic resistance: past, present and future. Int J Parasitol 29 : 115–124.
  7. Albonico M, Wright V, Bickle Q, 2004. Molecular analysis of the beta-tubulin gene of human hookworms as a basis for possible benzimidazole resistance on Pemba Island. Mol Biochem Parasitol 134 : 281–284.
  8. Reynoldson JA, Behnke JM, Pallant LJ, Macnish MG, Gilbert F, Giles S, Spargo RJ, Thompson RC, 1997. Failure of pyrantel in treatment of human hookworm infections (Ancylostoma duodenale) in the Kimberley region of northwest Australia. Acta Trop 68: 301–312.
  9. De Clercq D, Sacko M, Behnke J, Gilbert F, Dorny P, Vercruysse J, 1997. Failure of mebendazole in treatment of human hookworm infections in the southern region of Mali. Am J Trop Med Hyg 57 : 25–30.
  10. Geerts S, Gryseels B, 2000. Drug resistance in human helminths: current situation and lessons from livestock. Clin Microbiol Rev 13 : 207–222.
  11. Varady M, Corba J, 1999. Comparison of six in vitro tests in determining benzimidazole and levamisole resistance in Haemonchus contortus and Ostertagia circumcincta of sheep. Vet Parasitol 80 : 239–249.
  12. Taylor MA, Hunt KR, Goodyear KL, 2002. Anthelmintic resistance detection methods. Vet Parasitol 103 : 183–194.
  13. Palmer D, Mitchell T, Lyon J, 1998. Laboratory experiences with Drenchrite. Watts T, ed. Australian Sheep Veterinary Society AVA Conference. Sydney, 1–9.
  14. Folz SD, Pax RA, Thomas EM, Bennett JL, Lee BL, Condor GA, 1987. Motility response of several benzimidazole-resistant Haemonchus contortus larvae to several anthelmintics. Proc Helminthol Soc Wash 54 : 249–253.
  15. Gill JH, Redwin JM, van Wyk JA, Lacey E, 1991. Detection of resistance to ivermectin in Haemonchus contortus. Int J Parasitol 21 : 771–776.
  16. Behnke JM, Wells C, Brown J, 1986. An improved technique for experimental infections with skin penetrating nematode larvae (Necator americanus). Int J Parasitol 16 : 461–464.
  17. Garside P, Behnke JM, 1989. Ancylostoma ceylanicum in the hamster: observations on the host-parasite relationship during primary infection. Parasitology 98 : 283–289.
  18. Quinnell RJ, Griffin J, Nowell MA, Raiko A, Pritchard DI, 2001. Predisposition to hookworm infection in Papua New Guinea. Trans R Soc Trop Med Hyg 95 : 139–142.
  19. Garcia LS, 2001. Diagnostic Medical Parasitology. Washington, DC: American Society for Microbiology Press.
  20. Viney ME, Matthews BE, Walliker D, 1992. On the biological and biochemical nature of cloned populations of Strongyloides ratti. J Helminthol 66 : 45–52.
  21. Satou T, Koga M, Koike K, Tada I, Nikaido T, 2001. Nematocidal activities of thiabendazole and ivermectin against the larvae of Strongyloides ratti and S. venezuelensis. Vet Parasitol 99 : 311–322.
  22. Behnke JM, Rose R, Garside P, 1993. Sensitivity to ivermectin and pyrantel of Ancylostoma ceylanicum and Necator americanus. Int J Parasitol 23 : 945–952.
  23. Richards JC, Behnke JM, Duce IR, 1995. In vitro studies on the relative sensitivity to ivermectin of Necator americanus and Ancylostoma ceylanicum. Int J Parasitol 25 : 1185–1191.
  24. Colglazier ML, Kates KC, Enzie FD, 1975. Cross-resistance to other anthelmintics in an experimentally produced cambendazole-resistant strain of Haemonchus contortus in lambs. J Parasitol 61 : 778–779.
  25. Martin PJ, Anderson N, Jarrett RG, 1985. Resistance to benzimidazole anthelmintics in field strains of Ostertagia and Nematodirus in sheep. Aust Vet J 62 : 38–43.
  26. Elard L, Cabaret J, Humbert JF, 1999. PCR diagnosis of benzimidazole-susceptibility or -resistance in natural populations of the small ruminant parasite, Teladorsagia circumcincta. Vet Parasitol 80 : 231–237.
  27. Kwa MS, Veenstra JG, Roos MH, 1994. Benzimidazole resistance in Haemonchus contortus is correlated with a conserved mutation at amino acid 200 in beta-tubulin isotype 1. Mol Biochem Parasitol 63 : 299–303.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2004.71.608
Loading
/content/journals/10.4269/ajtmh.2004.71.608
Loading

Data & Media loading...

  • Received : 03 Feb 2004
  • Accepted : 11 May 2004

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error