Volume 71, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


While the response to is well characterized in mice, there is much less known about the human immune response, particularly early after exposure to the parasite. Therefore, we developed a primary (PIV) system that allowed us to address these questions. We co-cultured peripheral blood mononuclear cells from -naive donors with parasites and found that the responding PIV cells produced interferon-γ and interleukin-12 (IL-12). When restimulated, these PIV cells also occasionally produced IL-5. Both CD4 and CD8 cells and both HLA class I and II cell activation pathways appeared to play a role in the PIV system, and cell activation was dependent upon the presence of antigen-presenting cells. Moreover, PIV cells generated with showed considerable cross-reactivity with other species of . Finally, the PIV cells augmented intracellular killing of when they were co-cultured with macrophages infected with the parasite.


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  • Received : 22 Mar 2004
  • Accepted : 11 Jun 2004

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