Volume 71, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


. Giles Liston, Aitken, and Say are cultured and studied in molecular genetic and transgenic laboratories with increasing frequency. With limited research space, these mosquitoes are often maintained in the same insectary. Under these conditions, cross-contamination of colonies can occur and have devastating consequences to affected research programs. We have developed a polymerase chain reaction-based assay targeting the 28S large subunit ribosomal RNA gene to easily differentiate between these four taxa and Giles, which occurs in sympatry with . The resulting assay identifies individual mosquito preparations as well as all taxa within a mixed or pooled DNA template preparation.


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  • Received : 24 Dec 2003
  • Accepted : 14 Apr 2004
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