1921
Volume 71, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

. Giles Liston, Aitken, and Say are cultured and studied in molecular genetic and transgenic laboratories with increasing frequency. With limited research space, these mosquitoes are often maintained in the same insectary. Under these conditions, cross-contamination of colonies can occur and have devastating consequences to affected research programs. We have developed a polymerase chain reaction-based assay targeting the 28S large subunit ribosomal RNA gene to easily differentiate between these four taxa and Giles, which occurs in sympatry with . The resulting assay identifies individual mosquito preparations as well as all taxa within a mixed or pooled DNA template preparation.

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/content/journals/10.4269/ajtmh.2004.71.514
2004-10-01
2017-09-23
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References

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  7. Hiss RH, Norris DE, Dietrich CH, Whitcomb RF, West DF, Bosio CF, Kambhampati S, Piesman J, Antolin MF, Black WC IV, 1994. Molecular taxonomy using single strand conformation polymorphism (SSCP) analysis of mitochondrial ribosomal DNA genes. Insect Mol Biol 3 : 171–182.
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  • Received : 24 Dec 2003
  • Accepted : 14 Apr 2004

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