1921
Volume 71, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Classic techniques for detecting the susceptibility of to different drugs are time-consuming, laborious, and require the use of macrophages. The use of flow cytometry for monitoring susceptibility to drugs is beginning to be implemented. Using green fluorescent protein (GFP), we have improved and simplified the screening procedure. We introduced a GFP marker into field strains of causing kala-azar (visceral leishmaniasis) and explored the suitability of transgenic promastigotes that constitutively express GFP in their cytoplasm as target cells for screening of anti-leishmanial drugs.

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  • Received : 29 Jul 2003
  • Accepted : 09 Nov 2003

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