1921
Volume 71, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

is an emerging protozoan parasite capable of causing a protracted diarrheal illness in both immunocompromised and immunocompetent individuals. Ingestion of fresh produce and water sources contaminated with mature sporulated oocysts results in acquisition of cyclosporiasis. Currently, no animal model exists for the study of this pathogenic parasite and the only confirmed reservoir host for in nature is humans. Previously, -like oocysts had been detected by microscopy in several animals including non-human primates. However, their phylogenetic relationship to remained uncertain due to the limited availability of molecular techniques to differentiate and speciate these isolates. In the present study, we examined a series of fecal isolates obtained from dogs, chickens, and monkeys collected between May and September 2002 from several geographic regions of Nepal. All samples were examined by microscopy and a polymerase chain reaction (PCR) for the presence of Both microscopic and conventional PCR/restriction fragment length polymorphism (RFLP) analysis demonstrated the presence of sp. in the fecal samples of two dogs, one chicken, and one monkey. Application of a species-specific multiplex PCR assay confirmed the presence of both sp. and in the positive chicken sample and only in the dog and monkey samples. However, in the absence of tissue analysis, the assignment of these animals as a natural reservoir host for remains to be determined.

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2004-10-01
2017-09-24
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  • Received : 06 Oct 2003
  • Accepted : 14 Apr 2004

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