Volume 71, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Cysticercosis, a disease caused by the larval form of is diagnosed by detection of specific antibodies or by imaging techniques. Our preferred immunologic assay for cysticercosis is the enzyme-linked immunoelectrodifusion transfer blot, or immunoblot, using the lentil lectin bound antigens from larval cysts. Antibody reactivity with any one of seven glycoproteins is diagnostic for cysticercosis. To develop a simple antibody detection assay for field use, we have synthesized an 8-kD diagnostic antigen, sTs18var1 (a secreted protein with a mature size of 67 amino acids), and expressed a 50-kD membrane protein antigen, rGp50. We used these two diagnostic proteins in a quantitative Falcon assay screening test–enzyme-linked immunosorbent assay (FAST-ELISA) to measure the antibody responses in Peruvian pigs with cysticercosis. Three study designs were used. First, we followed the kinetics of antibody responses against these two diagnostic proteins in pigs with cysticercosis that were treated with oxfendazole. Second, we measured antibody response in naive experimentally infected pigs. Third, we followed the maternal antibodies against rGp50 and sTs18var1 in piglets born from sows with cysticercosis. These studies showed that antibody responses against the two diagnostic proteins in the FAST-ELISA are quantitatively correlated with infection by viable cysts, with anti-sTs18var1 activity being most responsive to the status of infection.


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  1. Garcia HH, Gilman R, Martinez M, Tsang VC, Pilcher JB, Herrera G, Diaz F, Alvarado M, Miranda E, 1993. Cysticercosis as a major cause of epilepsy in Peru. The Cysticercosis Working Group in Peru (CWG). Lancet 341 : 197–200. [Google Scholar]
  2. Garcia HH, Del Brutto OH, 2000. Taenia solium cysticercosis. Infect Dis Clin North Am 14 : 97–119. [Google Scholar]
  3. Gonzalez AE, Gilman R, Garcia HH, McDonald J, Kacena K, Tsang VC, Pilcher JB, Suarez F, Gavidia C, Miranda E, 1994. Use of sentinel pigs to monitor environmental Taenia solium contamination. The Cysticercosis Working Group in Peru (CWG). Am J Trop Med Hyg 51 : 847–850. [Google Scholar]
  4. Gonzalez AE, Verastegui M, Noh JC, Gavidia C, Falcon N, Bernal T, Garcia HH, Tsang VC, Gilman RH, Wilkins PP, 1999. Persistence of passively transferred antibodies in porcine Taenia solium cysticercosis. Cysticercosis Working Group in Peru. Vet Parasitol 86 : 113–118. [Google Scholar]
  5. Gonzalez AE, Gavidia C, Falcon N, Bernal T, Verastegui M, Garcia HH, Gilman RH, Tsang VC, Cysticercosis Working Group in Peru, 2001. Protection of pigs with cysticercosis from further infections after treatment with oxfendazole. Am J Trop Med Hyg 65 : 15–18. [Google Scholar]
  6. Tsang VC, Brand JA, Boyer AE, 1989. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159 : 50–59. [Google Scholar]
  7. Hancock K, Tsang VCW, 1986. Development and optimization of the FAST-ELISA for detecting antibodies to Schistosoma mansoni. J Immunol Methods 92 : 167–176. [Google Scholar]
  8. Richards FO, Tsang VCW, Brand JA, Hancock K, 1989. Modification of the FAST-ELISA for field diagnosis of Schistosomiasis mansoni with serum of blood samples. Trop Med Parasitol 40 : 332–334. [Google Scholar]
  9. Greene RM, Wilkins PP, Tsang VC, 1999. Diagnostic glycoproteins of Taenia solium cysts share homologous 14- and 18-kDa subunits. Mol Biochem Parasitol 99 : 257–261. [Google Scholar]
  10. Greene RM, Hancock K, Wilkins PP, Tsang VC, 2000. Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kDa related, diagnostic antigens. J Parasitol 86 : 1001–1007. [Google Scholar]
  11. Hancock K, Pattabhi S, Greene RM, Yushak ML, Williams F, Khan A, Priest JW, Levine MZ, Tsang VC, 2004. Characterization and cloning of GP50, a Taenia solium antigen diagnostic for cysticercosis. Mol Biochem Parasitol 133 : 115–124. [Google Scholar]
  12. Hancock K, Khan A, Williams FB, Yushak ML, Pattabhi S, Noh J, Tsang V, 2003. Characterization of the 8-kilodalton antigens of Taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis. J Clin Microbiol 41 : 2577–2586. [Google Scholar]
  13. Garcia HH, Gilman RH, Catacora M, Verastegui M, Gonzalez AE, Tsang VC, 1997. Serologic evolution of neurocysticercosis patients after antiparasitic therapy. J Infect Dis 175 : 486–489. [Google Scholar]
  14. Youden WJ, 1950. Index for rating diagnostic tests. Cancer 3 : 32–35. [Google Scholar]
  15. Elandt-Johnson RC, 1999. Survival Models and Data Analysis. New York: John Wiley & Sons, Inc.
  16. Xiao BG, Link H, 1998. Immune regulation within the central nervous system. J Neurol Sci 157 : 1–12. [Google Scholar]
  17. Garcia HH, Gonzalez AE, Gilman RH, Palacios LG, Jimenez I, Rodriguez S, Verastegui M, Wilkins P, Tsang VC; Cysticercosis Working Group in Peru, 2001. Short report: transient antibody response in Taenia solium infection in field conditions-a major contributor to high seroprevalence. Am J Trop Med Hyg 65 : 31–32. [Google Scholar]
  18. Garcia HH, Herrera G, Gilman RH, Tsang VC, Pilcher JB, Diaz JF, Candy EJ, Miranda E, Naranjo J, 1994. Discrepancies between cerebral computed tomography and Western blot in the diagnosis of neurocysticercosis. The Cysticercosis Working Group in Peru (Clinical Studies Coordination Board). Am J Trop Med Hyg 50 : 152–157. [Google Scholar]

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  • Received : 21 Aug 2003
  • Accepted : 01 Apr 2004

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