Volume 70, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Random amplified polymorphic DNA (RAPD) was used to detect intraspecific diversity for the complex. Fifty-two decameric to 21-meric primers of arbitrary sequence were applied to 15 strains that belong to nine zymodemes. Strains belonging to the species and were used as outgroups. A total of 902 amplicons generated by RAPD were scored. Most primers produced species-specific profiles, only 0.6% amplicons were shared by all species, while 4.3% amplicons were common for all 15 strains of the complex. Well-supported trees have been constructed, which show a rather strong correlation between the genetic polymorphism of studied strains and their geographic origin. In all obtained trees was paraphyletic. The RAPD profiles suggest that MON-30 belongs to Moreover, the genetic distance between the strain and other leishmanias does not warrant existence of a separate species.


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  • Received : 30 May 2003
  • Accepted : 22 Jan 2004

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