Volume 70, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


We present a polymerase chain reaction-restriction fragment length polymorphism method to simultaneously distinguish the two M and S molecular forms and . This method uses different diagnostic sites than previously published methods, and it is based on the amplification of a smaller ribosomal DNA fragment. We have tested this protocol in a variety of samples from different geographic regions and various ages of preservation to ascertain the robustness of this protocol over a wide geographic window and on DNA templates of poor quality. This procedure is as efficient as previous ones in discriminating from the two taxa in s.s. However, it performs better than others on poor quality templates such as the ones from museum collections, and poorly stored field collected material. However, it must be noted that it does not allow the simultaneous discrimination of all the species in the complex.


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  • Received : 26 Nov 2003
  • Accepted : 22 Jan 2004

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