1921
Volume 70, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Permethrin and DDT resistance in associated with a leucine-serine knockdown resistance () mutation in the voltage-gated sodium channel gene was discovered recently in western Kenya where a large scale permethrin-impregnated bed net (ITN) program has been implemented. Collections of were made from intervention and control villages prior to and after onset of the program. The genotypes were determined using allele-specific polymerase chain reaction diagnostic tests. In , the frequency of the mutation prior to ITN introduction was ~3–4% in western Kenya and zero in samples from the coast. After ITN introduction, the mutation increased in ITN and neighboring villages from ~4% to ~8%, but remained unchanged in villages at least 20 km distant and was not detected in coastal Kenya. The identical leucine-serine mutation was found in a single individual among 658 tested. The leucine-phenylalanine mutation common in west African populations was not detected in from Kenya. Implications for the population structure and control of are discussed.

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2004-06-01
2017-11-23
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  • Received : 31 Aug 2003
  • Accepted : 10 Oct 2003

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