1921
Volume 70, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The aim of this study was to determine the predominant hepatitis B virus (HBV) genotype in the Solomon Islands and determine if there is any racial correlation between genotype and hepatitis B e antigen (HBeAg) production in Japanese and Melanesian individuals. A total of 403 serum samples from 206 Melanesian HBV carriers in the Solomon Islands and 197 Japanese carriers from Fukuoka (n = 106) and Okinawa (n = 91) living in Japan in 2001 were tested. The HBV genotypes of 206 Melanesian subjects were 114 with genotype C (55.3%) and 92 with genotype D (44.7%). The HBV genotypes of 197 Japanese subjects were 74 with genotype B (37.6%) and 123 with genotype C (62.4%). The total HBeAg prevalence of subjects in Fukuoka (36.8%) was significantly higher than that of subjects in Okinawa (14.3%) ( < 0.0001) and subjects in the Solomon Islands (35.0%; = 0.0014, by the Mantel-Haenszel test). The genotype C prevalences were significantly different, ranging from 24.2% in Okinawa, to 54.4% in the Solomon Islands, to 95.3% in Fukuoka (all < 0.0001, by chi-square test). The prevalence of HBeAg positivity was significantly higher in Melanesian genotype C subjects (42.0%) than Melanesian genotype D subjects (26.6%) ( = 0.0310). Similarly, the prevalence of HBeAg positivity was significantly higher in Japanese genotype C subjects (36.6%) than Japanese genotype B subjects (9.5%) ( < 0.0001). These findings indicate that that HBV was of genotypes C and D in the Solomon Islands, and that the pathogenesis of HBV-infected patients is related to HBV genotype rather than race.

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2004-05-01
2017-11-22
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References

  1. Maynard JE, 1990. Hepatitis B: global importance and need for control. Vaccine 8 (suppl): S18–S20.
  2. Lai CL, 1997. Chronic hepatitis B in Hong Kong: immunization strategies for the control of hepatitis B virus infection. Zuckerman AJ, ed. Hepatitis B in the Asian-Pacific Region. Screening, Diagnosis and Control. Volume 1. London: Royal College of Physicians of London, 79–87.
  3. Gust ID, 1996. Epidemiology of hepatitis B infection in the western Pacific and south east Asia. Gut 38 (suppl 2): S18–S23.
  4. Furusyo N, Hayashi J, Kakuda K, Sawayama Y, Ariyama I, Eddie R, Kashiwagi S, 1999. Markedly high seroprevalence of hepatitis B virus infection in comparison to hepatitis C virus and human T lymphotropic virus type-1 infections in selected Solomon Islands populations. Am J Trop Med Hyg 61 : 85–91.
  5. Le Bouvier GL, McCollum RW, Hierholzer WJ Jr, Irwin GR, Krugman S, Giles JP, 1972. Subtypes of Australia antigen and hepatitis-B virus. JAMA 222 : 928–930.
  6. Shiina S, Fujino H, Kawabe T, Tagawa K, Unuma T, Yoneyama M, Ohmori T, Suzuki S, Kurita M, Ohashi Y, 1991. Relationship of HBsAg subtypes with HBeAg/anti-HBe status and chronic liver disease. Part II: Evaluation of epidemiological factors and suspected risk factors of liver dysfunction. Am J Gastroenterol 86 : 872–875.
  7. Noguchi A, Hayashi J, Nakashima K, Hirata M, Ikematsu H, Kashiwagi S, 1994. HBsAg subtypes among HBsAg carriers in Okinawa, Japan. Evidence of an important relationship in seroconversion from HBeAg to anti-HBe. J Infect 28 : 141–150.
  8. Furusyo N, Hayashi J, Sawayama Y, Kishihara Y, Kashiwagi S, 1999. Hepatitis B surface antigen disappearance and hepatitis B surface antigen subtype: a prospective, long-term, follow-up study of Japanese residents of Okinawa, Japan with chronic hepatitis B virus infection. Am J Trop Med Hyg 60 : 616–622.
  9. Okamoto H, Tsuda F, Sakugawa H, Sastrosoewignjo RI, Imai M, Miyakawa Y, Mayumi M, 1988. Typing hepatitis B virus by homology in nucleotide sequence: comparison of surface antigen subtypes. J Gen Virol 69 : 2575–2583.
  10. Norder H, Courouce AM, Magnius LO, 1994. Complete genomes, phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology 198 : 489–503.
  11. Stuyver L, De Gendt S, Van Geyt C, Zoulim F, Fried M, Schinazi RF, Rossau R, 2000. A new genotype of hepatitis B virus: complete genome and phylogenetic relatedness. J Gen Virol 81 : 67–74.
  12. Mayerat C, Mantegani A, Frei PC, 1999. Does hepatitis B virus (HBV) genotype influence the clinical outcome of HBV infection? J Viral Hepat 6 : 299–304.
  13. Telenta PF, Poggio GP, Lopez JL, Gonzalez J, Lemberg A, Campos RH, 1997. Increased prevalence of genotype F hepatitis B virus isolates in Buenos Aires, Argentina. J Clin Microbiol 35 : 1873–1875.
  14. Kao JH, Chen PJ, Lai MY, Chen DS, 2000. Hepatitis B genotypes correlate with clinical outcomes in patients with chronic hepatitis B. Gastroenterology 118 : 554–559.
  15. Orito E, Mizokami M, Sakugawa H, Michitaka K, Ishikawa K, Ichida T, Okanoue T, Yotsuyanagi H, Iino S, 2001. A case-control study for clinical and molecular biological differences between hepatitis B viruses of genotypes B and C. Hepatology 33 : 218–223.
  16. Furusyo N, Nakashima H, Kashiwagi K, Kubo N, Hayashida K, Usuda S, Mishiro S, Kashiwagi S, Hayashi J, 2002. Clinical outcomes of hepatitis B virus (HBV) genotypes B and C in Japanese patients with chronic HBV infection. Am J Trop Med Hyg 67 : 151–157.
  17. Grandjacques C, Pradat P, Stuyver L, Chevallier M, Chevallier P, Pichoud C, Maisonnas M, Trepo C, Zoulim F, 2000. Rapid detection of genotypes and mutations in the pre-core promoter and the pre-core region of hepatitis B virus genome: correlation with viral persistence and disease severity. J Hepatol 33 : 430–439.
  18. Sanchez-Tapias JM, Costa J, Mas A, Bruguera M, Rodes J, 2002. Influence of hepatitis B virus genotype on the long-term outcome of chronic hepatitis B in western patients. Gastroenterology 123 : 1848–1856.
  19. Nakashima H, Furusyo N, Kubo N, Kashiwagi K, Etoh Y, Kashiwagi S, Hayashi J, 2003. A double point mutation in the core promoter region of hepatitis B virus (HBV) genotype C may be related to liver deterioration in patients with chronic HBV infection. J Gastroenerol Hepatol (in press).
  20. Williams H, Mazzur S, 1976. Hepatitis B antigen subtypes in asymptomatic carriers in the Solomon Islands. Aust N Z J Med 6 : 210–213.
  21. Furusyo N, Hayashi J, Sawayama Y, Kawakami Y, Kishihara Y, Kashiwagi S, 1998. The elimination of hepatitis B virus infection: changing seroepidemiology of hepatitis A and B virus infection in Okinawa, Japan over a 26-year period. Am J Trop Med Hyg 59 : 693–698.
  22. Usuda S, Okamoto H, Iwanari H, Baba K, Tsuda F, Miyakawa Y, Mayumi M, 1999. Serological detection of hepatitis B virus genotypes by ELISA with monoclonal antibodies to type-specific epitopes in the preS2-region product. J Virol Methods 80 : 97–112.
  23. Usuda S, Okamoto H, Tanaka T, Kidd-Ljunggren K, Holland PV, Miyakawa Y, Mayumi M, 2000. Differentiation of hepatitis B virus genotypes D and E by ELISA using monoclonal antibodies to epitopes on the preS2-region product. J Virol Methods 87 : 81–89.
  24. Kato H, Orito E, Sugauchi F, Ueda R, Gish RG, Usuda S, Miyakawa Y, Mizokami M, 2001. Determination of hepatitis B virus genotype G by polymerase chain reaction with hemi-nested primers. J Virol Methods 98 : 153–159.
  25. Norder H, Hammas B, Lee SD, Bile K, Courouce AM, Mushahwar IK, Magnius LO, 1993. Genetic relatedness of hepatitis B viral strains of diverse geographical origin and natural variations in the primary structure of the surface antigen. J Gen Virol 74 : 1341–1348.
  26. Magnius LO, Norder H, 1995. Subtypes, genotypes and molecular epidemiology of the hepatitis B virus as reflected by sequence variability of the S-gene. Intervirology 38 : 24–34.
  27. Sugauchi F, Orito E, Ichida T, Kato H, Sakugawa H, Kakumu S, Ishida T, Chutaputti A, Lai CL, Ueda R, Miyakawa Y, Mizokami M, 2002. Hepatitis B virus of genotype B with or without recombination with genotype C over the precore region plus the core gene. J Virol 76 : 5985–5992.
  28. Sugauchi F, Orito E, Ichida T, Kato H, Sakugawa H, Kakumu S, Ishida T, Chutaputti A, Lai CL, Gish RG, Ueda R, Miyakawa Y, Mizokami M, 2003. Epidemiologic and virologic characteristics of hepatitis B virus genotype B having the recombination with genotype C. Gastroenterology 124 : 925–932.
  29. Kidd-Ljunggren K, Miyakawa Y, Kidd AH, 2002. Genetic variability in hepatitis B viruses. J Gen Virol 83 : 1267–1280.
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  • Received : 04 Jun 2003
  • Accepted : 12 Oct 2003

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