1921
Volume 70, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Genotypes of causing disease were investigated in pulmonary tuberculosis patients admitted to two adjacent wards of a tuberculosis hospital in Delhi, India. Genetic markers, the insertion sequence IS6110, a direct repeat sequence, and a polymorphic GC-rich sequence supported the circumstantial epidemiologic link between eight strains of , suggesting their possible involvement in small-scale, interpersonal transmission of both drug-sensitive and drug-resistant tuberculosis. This is the first report of a suspected acquisition of among hospitalized patients in India. The use of multiple molecular typing markers and techniques unequivocally identified the exact clonality of strains isolated from the hospital. The result of this study emphasizes the need for more comprehensive investigation of high-risk situations for tuberculosis transmission and long-term follow-up analysis for identifying such instances of unsuspected transmission.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2004.70.527
2004-05-01
2017-09-20
Loading full text...

Full text loading...

/deliver/fulltext/14761645/70/5/0700527.html?itemId=/content/journals/10.4269/ajtmh.2004.70.527&mimeType=html&fmt=ahah

References

  1. van Soolingen D, 2001. Molecular epidemiology of tuberculosis, and other mycobacterial infections: main methodologies, and achievements. J Intern Med 249 : 1–26.
  2. Couldwell DL, Dore GJ, Harkness JL, Marriott DJ, Cooper DA, Edwards R, Li Y, Kaldor JM, 1996. Nosocomial outbreak of tuberculosis in an outpatient HIV treatment room. AIDS 10 : 521–525.
  3. Breathnach AS, de Ruiter A, Holdsworth GM, Bateman NT, O’Sullivan DG, Rees PJ, Snashall D, Milburn HJ, Peters BS, Watson J, Drobniewski FA, French GL, 1998. An outbreak of multi-drug-resistant tuberculosis in a London teaching hospital. J Hosp Infect 39 : 111–117.
  4. Moro ML, Errante I, Infuso A, Sodano L, Gori A, Orcese CA, Salamina G, D’Amico C, Besozzi G, Caggese L, 2000. Effectiveness of infection control measures in controlling a nosocomial outbreak of multidrug-resistant tuberculosis among HIV patients in Italy. Int J Tuberc Lung Dis 4 : 61–68.
  5. Tudo G, Gonzalez J, Gatell JM, Cayla JA, Martinez E, Garcia A, Navarro M, Soriano E, Jimenez de Anta MT, and the Tuberculosis Investigation Unit of Barcelona, Spain, 2001.Detection of unsuspected cases of nosocomial transmission of tuberculosis by use of a molecular typing method. Clin Infect Dis 33 : 453–459.
  6. The WHO/IUATLD Global Project on Anti-Tuberculosis Drug Resistance Surveillance, 1994–1997. Geneva: World Health Organization.
  7. Udwadia ZF, 2001. India’s multidrug-resistant tuberculosis crisis. Ann N Y Acad Sci 953 : 98–105.
  8. Sahadevan R, Narayanan S, Paramasivam CN, Prabhakar R, Narayanan PR, 1995. Restriction fragment length polymorphism typing of clinical isolates of Mycobacterium tuberculosis from patients with pulmonary tuberculosis in Madras, India by use of direct-repeat probe. J Clin Microbiol 33 : 3037–3039.
  9. Harris KA Jr, Mukundan U, Musser JM, Kreiswirth BN, Lalitha MK, 2000. Genetic diversity, and evidence for acquired antimicrobial resistance in Mycobacterium tuberculosis at a large hospital in South India. Int J Infect Dis 4 : 140–147.
  10. Shriniwas, Bhatia VN, 1973.A study of mycobacterial species isolated from patients with chronic respiratory disease. Am Rev Resp Dis 108 : 378–381.
  11. van Embden JDA, Cave MD, Crawford JT, Dale JW, Eisenach KD Gicquel B, Hermans P, Martin C, McAdam R, Shinnick TM, Small PM, 1993. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol 31 : 406–409.
  12. Kamerbeek J, Schouls LM, van Agterveld M, van Soolingen D, Bunschoten AE, Kolk HJ, Kuijper S, van Embden JDA, 1997. Rapid detection, and simultaneous strain differentiation of Mycobacterium tuberculosis for diagnosis, and tuberculosis control. J Clin Microbiol 35 : 907–914.
  13. Valway SE, Sanchez MP, Shinnick TF, Orme I, Agerton J, Hoy D, Jones JS, Westmorel, and H, Onorato IM, 1998. An outbreak involving extensive transmission of a virulent strain of Mycobacterium tuberculosis. N Engl J Med 338 : 633–639.
  14. Bhanu NV, van Soolingen D, van Embden JDA, Dar L, P, Andey RM, Seth P, 2002. Predominace of a novel Mycobacterium tuberculosis genotype in the Delhi region of India. Tuberculosis (Edinb) 82 : 105–112.
  15. Thomas C, 1944. Exogenous or endogenous reinfection: is an arrested primary infection protective or deleterious? Rich AR, ed. The Pathogenesis of Tuberculosis, Springfield, IL: 779–850.
  16. Stead WW, 1967. Pathogenesis of a first episode of chronic pulmonary tuberculosis in man: recrudescnce of residuals of the primary infection or exogenous reinfection? Am Rev Respir Dis 95 : 729–745.
  17. Romeyn JA, 1970. Exogenous reinfection in tuberculosis. Am Rev Respir Dis 101 : 923–927.
  18. Nardell E, McInnis B, Thomas B, Weidhaas S, 1986. Exogenous reinfection with tuberculosis in a shelter for the homeless. N Engl J Med 315 : 1570–1575.
  19. CDC, 1991. Nosocomial transmission of multidrug resistant tuberculosis among HIV infected persons: Florida, and New York, 1986–1991. MMWR Morb Mortal Wkly Rep 40 : 535–590.
  20. CDC, 1993. Outbreak of multiple drug-resistant tuberculosis at a hospital-New York City, 1991. MMWR Morb Mortal Wkly Rep 42 : 427–435.
  21. Wallgren A, 1948. The time table of tuberculosis. Tubercle 29 : 245–251.
  22. British Medical Research Council, 1972. BCG, and vole bacillus in the prevention of tuberculosis in adolescence, and early adult life. Bull World Health Organ 46 : 371–385.
  23. Pablos-Méndez A, Blustein J, Knirsch CA, 1997. The role of diabetes mellitus in the higher prevalence of tuberculosis among Hispanics. Am J Public Health 87 : 574–579.
  24. Jacobson, 1992. Alcoholism, and tuberculosis. Alcohol Health Res World 16 : 39–47.
  25. Li X, Grossman CJ, Mendenhall CL, Hurtubise P, Rouster SD, Roselle GA, Gartside P, 1998. Host response to mycobacterial infection in the alcoholic rat: male, and female dimorphism. Alcohol 16 : 207–212.
  26. Maurya V, Vijayan VK, Shah A, 2002. Smoking, and tuberculosis: an association overlooked. Int J Tuberc Lung Dis 6 : 942–951.
  27. Kenyon TA, Creek T, Laserson K, Makhoa M, Chimidza N, Mwasekaga M, Tappero J, Lockman S, Moeti T, Binkin N, 2002. Risk factors for transmission of Mycobacterium tuberculosis from HIV-infected tuberculosis patients. Botswana Int J Tuberc Lung Dis. 6 : 843–850.
  28. Small PM, McClenny NB, Singh SP, Schoolnik GK, Tompkins LS, Mickelsen PA, 1993. Molecular strain typing of Mycobacterium tuberculosis to confirm cross-contamination in the my-cobacteriology laboratory, and modification of procedures to minimize occurrence of false-positive cultures. J Clin Microbiol. 31 : 1677–1682.
  29. Mastorides SM, Oehler RL, Greene JN, Sinnott JT IV, Kranik M, S, Andin RL, 2001. The detection of airborne Mycobacterium tuberculosis using micropore membrane air sampling, and polymerase chain reaction. Chest 115 : 19–25.
  30. CDC, 1994. Guidelines for preventing the transmission of Myco-bacterium tuberculosis in health-care facilities. MMWR Morb Mortal Wkly Rep 43 : 1–132.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2004.70.527
Loading
/content/journals/10.4269/ajtmh.2004.70.527
Loading

Data & Media loading...

  • Received : 24 Mar 2003
  • Accepted : 03 Sep 2003

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error