Volume 70, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


The influence of several factors on parasite growth and 50% inhibitory concentration (IC) for chloroquine was assessed. Most isolates stored at 4°C up to 72 hours grew when they were subsequently cultivated. However, parasite viability sharply decreased from 24 hours, and the mean chloroquine IC decreased significantly ( < 0.05). There was no evidence for selection of pre-culture populations due to storage alone. The time point when H-hypoxanthine was added (0 versus 18 hours) had no effect on the IC during the 42-hour incubation, but was associated with a lower IC when H-hypoxanthine was added after the initial 42-hour incubation during the 72-hour incubation. An increase in 3H-hypoxanthine incorporation and chloroquine IC was observed as the hematocrit was increased from 1.0% to 2.5%. For the same isolates, chloroquine IC values were generally similar when the initial parasitemia was between 0.1% and 0.5% but increased at higher (>0.75%) parasitemias. Based on these results, we recommend immediate cultivation after blood collection, a 42-hour incubation period with the addition of H-hypoxanthine at the beginning of incubation, a 1.5% hematocrit, and an initial parasitemia 0.1-0.5%. Further studies on serum substitutes, gas mixture, and comparison of isotopic and non-isotopic assays are needed to establish a standardized assay protocol.


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  • Received : 13 Jul 2003
  • Accepted : 08 Jan 2004

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