1921
Volume 70, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Previous studies using methods varying from traditional serologic tests to molecular biology techniques have shown that in northeastern Brazil, was more prevalent than . In this study, the prevalence was established by using stool antigen detection kits and a polymerase chain reaction (PCR) with genomic DNA extracted from cultured trophozoites in all four-nuclei, amoeba-positive samples from a population living in Macaparana in northeastern Brazil. Among 1,437 stool samples analyzed, only 59 (4.1%) were positive for four nuclei amoeba. However, all of these samples were negative in an immunoenzymatic assay for the presence of -specific galactose adhesin. Of 59 cultivated samples, only 31 showed trophozoites. Extraction of DNA from these 31 samples, followed by the PCR, showed that 23 samples (74.19%) were positive for and no amplification was observed for pathogenic . The remaining eight samples were negative for both species. These findings are consistent with those previously reported.

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2004-02-01
2017-09-24
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  • Received : 14 Aug 2003
  • Accepted : 07 Oct 2003

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