1921
Volume 70, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

We evaluated the ICT Malaria immunochromatographic test for the detection of the panmalarial antigen (PMA) using a rodent malaria model. Mice were infected with by mosquito bite, and blood was examined by microscopy and the ICT test. Treatment with artemether was started when the parasite density exceeded 70,000/μL. The ICT PMA band appeared when the parasite density was more than 2,000/μL, but it continued to be positive after the parasitemia became negative in response to the drug treatment. When all the test results were divided into increasing phase (IP) and declining phase (DP), the sensitivity in the DP was significantly higher than that in the IP, suggesting that the reactivity of the ICT PMA is significantly influenced by persistent and accumulated PMA after drug treatment and longer duration of infection in the DP. Recognizing that the patient population in a clinical situation would be a mixture of individuals in the IP and DP, it should be emphasized that the individual history of recent fever, duration of illness, and drug treatment must be considered carefully for the interpretation of the ICT results.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2004.70.139
2004-02-01
2017-07-21
Loading full text...

Full text loading...

/deliver/fulltext/14761645/70/2/0700139.html?itemId=/content/journals/10.4269/ajtmh.2004.70.139&mimeType=html&fmt=ahah

References

  1. Moody A, 2002. Rapid diagnostic tests for malaria parasites. Clin Microbiol Rev 15 : 66–78.
  2. Tjitra E, Suprianto S, Dyer M, Currie BJ, Anstey NM, 1999. Field evaluation of the ICT malaria P.f/P.v immunochromatographic test for detection of Plasmodium falciparum and Plasmodium vivax in patients with a presumptive clinical diagnosis of malaria in eastern Indonesia. J Clin Microbiol 37 : 2412–2417.
  3. Mason DP, Wongsrichanalai C, Lin K, Miller RS, Kawamoto F, 2001. The panmalarial antigen detected by the ICT Malaria P.f/P.v immunochromatographic test is expressed by Plasmodium malariae. J Clin Microbiol 39 : 2035.
  4. Win TT, Tantular IS, Pusarawati S, Kerong H, Lin K, Matsuoka H, Ishii A, Kawamoto F, 2001. Detection of Plasmodium ovale by the ICT malaria P.f/P.v. immunochromatographic test. Acta Trop 80 : 283–284.
  5. Grobusch MP, Hanscheid T, Zoller T, Jelinek T, Burchard GD, 2002. Rapid immunochromatographic malarial antigen detection unreliable for detecting Plasmodium malariae and Plasmodium ovale. Eur J Clin Microbiol Infect Dis 21 : 818–820.
  6. Valecha N, Eapen A, Usha Devi C, Ravindran J, Aggarwal A, Subbarao SK, 2002. Field evaluation of the ICT Malaria P.f./P.v. immunochromatographic test in India. Ann Trop Med Parasitol 96 : 333–336.
  7. Shiff CJ, Premji Z, Minjas JN, 1993. The rapid manual ParaSight-F test. A new diagnostic tool for Plasmodium falciparum infection. Trans R Soc Trop Med Hyg 87 : 646–648.
  8. Miller RL, Ikram S, Armelagos GJ, Walker R, Harer WB, Shiff CJ, Baggett D, Carrigan M, Maret SM, 1994. Diagnosis of Plasmodium falciparum infections in mummies using the rapid manual ParaSight-F test. Trans R Soc Trop Med Hyg 88 : 31–32.
  9. Karbwang J, Tasanor O, Kanda T, Wattanagoon Y, Ibrahim M, Na-Bangchang K, Thanavibul A, Rooney W, 1996. ParaSight-F test for the detection of treatment failure in multidrug resistant Plasmodium falciparum malaria. Trans R Soc Trop Med Hyg 90 : 513–515.
  10. Laferi H, Kandel K, Pichler H, 1997. False positive dipstick test for malaria. N Engl J Med 337 : 1635–1636.
  11. Humar A, Ohrt C, Harrington MA, Pillai D, Kain KC, 1997. Parasight F test compared with the polymerase chain reaction and microscopy for the diagnosis of Plasmodium falciparum malaria in travelers. Am J Trop Med Hyg 56 : 44–48.
  12. Eisen DP, Saul A, 2000. Disappearance of panmalarial antigen reactivity using the ICT Malaria P.f/P.v kit parallels decline of patent parasitaemia as shown by microscopy. Trans R Soc Trop Med Hyg 94 : 169–170.
  13. Tjitra E, Suprianto S, McBroom J, Currie BJ, Anstey NM, 2001. Persistent ICT malaria P.f/P.v panmalarial and HRP2 antigen reactivity after treatment of Plasmodium falciparum malaria is associated with gametocytemia and results in false-positive diagnoses of Plasmodium vivax in convalescence. J Clin Microbiol 39 : 1025–1031.
  14. Tjitra E, Suprianto S, Dyer ME, Currie BJ, Anstey NM, 2001. Detection of histidine rich protein 2 and panmalarial ICT Malaria Pf/Pv test antigens after chloroquine treatment of uncomplicated falciparum malaria does not reliably predict treatment outcome in eastern Indonesia. Am J Trop Med Hyg 65 : 593–598.
  15. Iqbal J, Khalid N, Hira PR, 2002. Comparison of two commercial assays with expert microscopy for confirmation of symptomatically diagnosed malaria. J Clin Microbiol 40 : 4675–4678.
  16. Matsuoka H, Yoshida S, Hirai M, Ishii A, 2002. A rodent malaria, Plasmodium berghei, is experimentally transmitted to mice by merely probing of infective mosquito, Anopheles stephensi. Parasitol Int 51 : 17–23.
  17. Coleman RE, Maneechai N, Rachapaew N, Kumpitak C, Soyseng V, Miller RS, Thimasarn K, Sattabongkot J, 2002. Field evaluation of the ICT Malaria Pf/Pv immunochromatographic test for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand. Am J Trop Med Hyg 66 : 379–383.
  18. Bechem NN, Leke RF, Tietche F, Taylor DW, 1999. Evaluation of a rapid test for histidine rich protein 2 for diagnosis of Plasmodium falciparum infection in Cameroonian children. Trans R Soc Trop Med Hyg 93 : 46.
  19. Singh N, Saxena A, Valecha N, 2000. Field evaluation of the ICT Malaria P.f/P.v immunochromatographic test for diagnosis of Plasmodium falciparum and P.vivax infection in forest villages of Chhindwara, central India. Trop Med Int Health 5 : 765–770.
  20. Huong NM, Davis TME, Hewitt S, Huong NV, Uyen TT, Nhan DH, Cong LD, 2002. Comparison of three antigen detection methods for diagnosis and therapeutic monitoring of malaria: a field study from southern Vietnam. Trop Med Int Health 7 : 304–308.
  21. Playford EG, Walker J, 2002. Evaluation of the ICT malaria P.f/P.v and the OptiMal rapid diagnostic tests for malaria in febrile returned travellers. J Clin Microbiol 40 : 4166–4171.
  22. Smith T, Felger I, Tanner M, Beck HP, 1999. Premunition in Plasmodium falciparum infection: insights from the epidemiology of multiple infections. Trans R Soc Trop Med Hyg 93 (Suppl 1): 59–64.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2004.70.139
Loading
/content/journals/10.4269/ajtmh.2004.70.139
Loading

Data & Media loading...

  • Received : 17 Jul 2003
  • Accepted : 07 Oct 2003

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error