Volume 70, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


This paper describes the development of a novel microfluorimetric assay to measure the inhibition of based on the detection of parasitic DNA by intercalation with PicoGreen. The method was used to determine parasite inhibition profiles and 50% inhibitory concentration values of known or potential antimalarial drugs. Values for parasite inhibition with known antimalarial drugs using the PicoGreen assay were comparable with those determined by the standard method based upon the uptake of H-hypoxanthine and the Giemsa stain microscopic technique. The PicoGreen assay is rapid, sensitive, reproducible, easily interpreted, and ideally suited for screening of large numbers of samples for antimalarial drug development.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...



  1. Greenwood B, Mutabingwa T, 2002. Malaria in 2002. Nature 415 : 670–672. [Google Scholar]
  2. Riddley RG, 1999. Planting the seeds of new antimalarial drugs. Science 285 : 1502–1503. [Google Scholar]
  3. Desjardins RE, Canfield CJ, Haynes JD, Chulay JD, 1979. Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique. Antimicrob Agents Chemother 16 : 710–718. [Google Scholar]
  4. Makler MT, Gibbins BL, 1991. Laboratory diagnosis of malaria. Clin Lab Med 11 : 941–956. [Google Scholar]
  5. Delhaes L, Lazaro JE, Gay F, Thellier M, Danis M, 1999. The microculture tetrazolium assay (MTA): another colorimetric method of testing Plasmodium falciparum chemosensitivity. Annals Trop Med Parasitol 93 : 31–40. [Google Scholar]
  6. Makler MT, Hinrichs DJ, 1993. Measurement of the lactate dehydrogenase activity of Plasmodium falciparum as an assessment of parasitemia. Am J Trop Med Hyg 48 : 205–210. [Google Scholar]
  7. Klayman DL, 1993. Artemisia annua, from weed to respectable antimalarial plant. Kinghorn AD, Balandron MA, eds. Human Medicinal Agents from Plants. Washington, DC: American Chemical Society 242–255.
  8. Muñoz V, Sauvain M, Bourdy G, Callapa J, Bergeron S, Rojas I, Bravo JA, Balderrama L, Ortiz B, Gimenez A, DeHaro E, 2000. A search for natural bioactive compounds in Bolivia through a multidisciplinary approach Part I. Evaluation of the antimalarial activity of plants used by the Chacobo Indians. J Ethnopharmacol 69 : 127–137. [Google Scholar]
  9. Kursar TA, Capson TL, Coley PD, Corley DG, Gupta MB, Harrison LA, Ortega-Barría E, Windsor DM, 1999. Ecologically guided bioprospecting in Panama. Pharmaceut Biol 37 (suppl): 114–126. [Google Scholar]
  10. Trager W, Jensen JB, 1976. Human malaria parasites in continuous culture. Science 193 : 673–675. [Google Scholar]
  11. Lambros C, Vanderberg JP, 1979. Synchronization of Plasmodium falciparum erythrocytic stages in culture. J Parasitol 65 : 418–420. [Google Scholar]
  12. Montenegro H, Gutiérrez M, Romero LI, Ortega-Barría E, Capson TL, Cubilla-Rios L, 2003. Aporphine alkaloids from Guatteria spp. with leishmanicidal activity. Planta Med 69 : 677–679. [Google Scholar]
  13. DeHaro E, Gautret P, Munoz V, Sauvain M, 2000. Evaluación de la actividad antimalarica in vitro de productos naturales o de sintesis. Técnicas de Laboratorio para la Selección de Sustancias Antimalaricas. CYTED. La Paz, Bolivia: Imprenta Perez, 51–88.
  14. Basco LK, Marquet F, Makler MT, Le Bras J, 1995. Plasmodium falciparum and Plasmodium vivax: Lactate dehydrogenase activity and its application for in vitro drug susceptibility assay. Exp Parasitol 80 : 260–271. [Google Scholar]
  15. Makler MT, Ries JM, Williams JA, Bancroft JE, Piper RC, Gibbins BL, Hinrichs DJ, 1993. Parasite lactate dehydrogenase as an assay for Plasmodium falciparum drug sensitivity. Am J Trop Med Hyg 48 : 739–741. [Google Scholar]
  16. Westenburg HE, Lee KJ, Lee SK, Fong HHS, Van Breemen RB, Pezzuto JM, Kinghorn DA, 2000. Activity-guided isolation of antioxidative constituents of Cotinus coggygria. J Nat Prod 63 : 1696–1698. [Google Scholar]
  17. Smeijsters LJJW, Zijlstra NM, Franssen FFJ, Overdule JP, 1996. Simple, fast, and accurate fluorimetric method to determine drug susceptibility of Plasmodium falciparum in 24-well suspension cultures. Antimicrobial Agents Chemother 40 : 835–838. [Google Scholar]

Data & Media loading...

  • Received : 04 Apr 2003
  • Accepted : 01 Oct 2003

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error