Volume 69, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


The development of comparative genetic maps in multiple species of mosquitoes could prove extremely useful in the search for those genes that contribute to mosquito vector competence or genes associated with other phenotypes of interest. To effectively compare these gene maps, markers must be developed that are based on chromosomal regions conserved throughout the Culicidae. We designed 35 polymerase chain reaction (PCR) primer pairs based upon orthlogous exons in and or . Twenty-three of the primers yielded a single PCR product in at least one dipteran, in addition to , when screened with genomic DNA from seven dipterans, including five mosquito species. Eight of the primers amplified a single PCR product in only , while four primer pairs gave no PCR product in any species. The 23 successful comparative anchor-tagged sequence primer pairs give broad genome coverage in , and more importantly demonstrate an efficient strategy for developing comparative anchor marker loci for any species of Culicidae.


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  1. Knight KL, Stone A, 1977. A Catalog of the Mosquitoes of the World (Diptera:Culicidae). Baltimore, MD: The Thomas Say Foundation.
  2. Munstermann LE, Craig GB Jr, 1979. Genetics of Aedes aegypti: updating the linkage map. J Hered 70 : 291–296.
  3. O’Brien SJ, 1990. Genetic Maps: Locus Maps of Complex Genomes: Lower Eukaryotes. Fifth edition. Volume 3. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  4. O’Brien SJ, 1993. Genetic Maps: Locus Maps of Complex Genomes: Lower Eukaryotes (33 Maps). Sixth edition. Volume 3. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  5. Matthews TC, Munstermann LE, 1994. Chromosomal repatterning and linkage group conservation in mosquito karyotypic evolution. Evolution 48 : 146–154.
  6. Severson DW, Mori A, Zhang Y, Christensen BM, 1993. Linkage map for Aedes aegypti using restriction fragment length polymorphisms. J Hered 84 : 241–247.
  7. Severson DW, Meece JK, Lovin DD, Saha G, Morlais I, 2002. Linkage map organization of expressed sequence tags and sequence tagged sites in the mosquito Aedes aegypti. Insect Mol Biol 11 : 371–378.
  8. Antolin MF, Bosio CF, Cotton J, Sweeney W, Strand MR, Black WC IV, 1996. Intensive linkage mapping in a wasp (Bracon hebetor) and a mosquito (Aedes aegypti) with single-strand conformation polymorphism analysis of random amplified polymorphic DNA markers. Genetics 143 : 1727–1738.
  9. Fulton RE, Salasek ML, DuTeau NM, Black WC IV, 2001. SSCP analysis of cDNA markers provides a dense linkage map of the Aedes aegypti genome. Genetics 158 : 715–726.
  10. Wang R, Kafatos FC, Zheng L, 1999. Microsatellite markers and genotyping procedures for Anopheles gambiae. Parasitol Today 15 : 33–37.
  11. Severson DW, Mori A, Zhang Y, Christensen BM, 1994. Chromosomal mapping of two loci affecting filarial worm susceptibility in Aedes aegypti. Insect Mol Biol 3 : 67–72.
  12. Severson DW, Thathy V, Mori A, Zhang Y, Christensen BM, 1995. Restriction fragment length polymorphism mapping of quantitative trait loci for malaria parasite susceptibility in the mosquito Aedes aegypti. Genetics 139 : 1711–1717.
  13. Beerntsen BT, Severson DW, Klinkhammer JA, Kassner VA, Christensen BM, 1995. Aedes aegypti: a quantitative trait locus (QTL) influencing filarial worm intensity is linked to QTL for susceptibility to other mosquito-borne pathogens. Exp Parasitol 81 : 355–362.
  14. Zheng L, Cornel AJ, Wang R, Erfle H, Voss H, Ansorge W, Kafatos FC, Collins FH, 1997. Quantitative trial loci for refractoriness of Anopheles gambiae to Plasmodium cynomolgi B. Science. 276 : 425–428.
  15. Gorman MJ, Severson DW, Cornel AJ, Collins FH, Paskewitz SM, 1997. Mapping a quantitative trait locus involved in melanotic encapsulation of foreign bodies in the malaria vector, Anopheles gambiae. Genetics 146 : 965–971.
  16. Bosio CF, Fulton RE, Salasek ML, Beaty BJ, Black WC IV, 2000. Quantitative trait loci that control vector competence for dengue-2 virus in the mosquito Aedes aegypti. Genetics 156 : 687–698.
  17. Mori A, Severson DW, Christensen BM, 1999. Comparative linkage maps for the mosquitoes (Culex pipiens and Aedes aegypti) based on common RFLP loci. J Hered 90 : 160–164.
  18. Severson DW, Mori A, Kassner VA, Christensen BM, 1995. Comparative linkage maps for the mosquitoes Aedes albopictus and Ae. aegypti, based on common RFLP loci. Insect Mol Biol 4 : 41–45.
  19. Anderson JR, Grimstead PR, Severson DW, 2001. Chromosomal evolution among six mosquito species (Diptera: Culicidae) based upon shared restriction fragment length polymorphisms. Mol Phylogenet Evol 20 : 316–321.
  20. Mori A, Tomita T, Hidoh O, Kono Y, Severson DW, 2001. Comparative linkage map development and identification of an autosomal locus for insensitive acetylcholinesterase-mediated insecticide resistance in Culex tritaeniorhynchus. Insect Mol Biol 10 : 197–203.
  21. Ferdig MT, Taft AS, Severson DW, Christensen BM, 1998. Development of a comparative genetic linkage map for Armigeres subalbatus using Aedes aegypti RFLP markers. Genome Res 8 : 41–47.
  22. Severson DW, Mori A, Zhang Y, Christensen BM, 1994. The suitability of restriction fragment length polymorphism markers for evaluating genetic diversity among and synteny between mosquito species. Am J Trop Med Hyg 50 : 425–432.
  23. Lyons LA, Laughlin TF, Copeland NG, Jenkins NA, Womack JE, O’Brien SJ, 1997. Comparative anchor tagged sequences (CATS) for integrative mapping of mammalian genomes. Nat Genet 15 : 47–56.
  24. Smith E, Shi L, Drummond P, Rodriguez L, Hamilton R, Powell E, Nahashon S, Ramlal S, Smith G, Foster J, 2000. Development and charaterization of expressed sequence tags for the turkey (Meleagris gallopavo) genome and comparative sequence analysis with other birds. Anim Genet 31 : 62–67.
  25. Mazzarella R, Montanaro V, Kere J, Reinbold R, Ciccodicola A, D’Urso M, Schlessinger D, 1992. Conserved sequence-tagged sites: a phylogenetic approach to genome mapping. Proc Natl Acad Sci USA 89 : 3681–3685.
  26. Caetano AR, Lyons LA, Laughlin TF, O’Brien SJ, Murray JD, Bowling AT, 1999. Equine synteny mapping of comparative anchor tagged sequences (CATS) from human chromosome 5. Mamm Genome 10 : 1082–1084.
  27. Chen Z.-Q, Lautenberger JA, Lyons LA, McKenzie L, O’Brien SJ, 1999. A human genome map of comparative anchor tagged sequences. J Hered 90 : 477–484.
  28. Lyons LA, Kehler JS, O’Brien SJ, 1999. Development of comparative anchor tagged sequences (CATS) for canine genome mapping. J Hered 90 : 15–26.
  29. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ, 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25 : 3389–3402.
  30. Smith RF, Wiese BA, Wojzynski MK, Davison DB, Worley KC, 1996. BCM Search Launcheran integrated interface to molecular biology data base search and analysis services available on the World Wide Web. Genome Res 6 : 454–462.
  31. Severson DW, 1997. RFLP analysis of insect genomes. Crampton JM, Beard CB, and Louis C, eds. The Molecular Biology of Insect Disease Vectors: A Methods Manual. London: Chapman and Hall: 309–320.
  32. Tatusova TA, Madden TL, 1999. Blast2 sequences - a new tool for comparing protein and nucleotide sequences. FEMS Microbiol Lett 174 : 247–250.
  33. van Ooijen JW, 1994. DrawMap: a computer program for drawing genetic linkage maps. J Hered 85 : 66.
  34. World Health Organization, 1997. Division of Control of Tropical Diseases. Available on the World Wide Web at URL http://www.who.ch.
  35. Hiss RH, Norris DE, Dietrich CH, Whitcomb RF, West DF, Bosio CF, Kambhampati S, Piesman J, Antolin MF, Black WC IV, 1994. Molecular taxonomy using single-strand conformation polymorphism (SSCP) analysis of mitochondrial ribosomal DNA genes. Insect Mol Biol 3 : 171–182.
  36. Akey JM, Sosnoski D, Parra E, Dios S, Hiester K, Su B, Bonilla C, Jin L, Shriver MD, 2001. Melting curve analysis of SNPs (McSNP): a gel free and inexpensive approach for SNP genotyping. Biotechniques 30 : 358–367.
  37. Weller GL, Foster GG, 1993. Genetic maps of the sheep blowfly Lucilia cuprina: linkage group correlations with other dipteran genera. Genome 36 : 495–506.
  38. Ranz JM, Caceres M, Ruiz A, 1999. Comparative mapping of cosmids and gene clones from a 1.6 Mb chromosomal region of Drosophila melanogaster in three species of distantly related subgenus Drosophila. Chromosoma 108 : 32–43.
  39. Ranz JM, Casals F, Ruiz A, 2001. How malleable is the eukaryotic genome? Extreme rate of chromosomal rearrangement in the genus Drosophila. Genet Res 11 : 230–239.
  40. Bolshakov VN, Topalis P, Blass C, Kokoza E, della Torre A, Kafatos FC, Louis C, 2002. A comparative genomic analysis of two distant Diptera, the fruit fly, Drosophila melanogaster, and the malaria mosquito, Anopheles gambiae. Genet Res 12 : 57–66.
  41. Zdobnov EM, von Mering C, Letunic I, Torrents D, Suyama M, Copley RR, Christophides GK, Thomasova D, Holt RA, Subramanian GM, Mueller HM, Dimopoulos G, Law JH, Wells MA, Birney E, Charlab R, Halpern AL, Kokoza E, Kraft CL, Lai Z, Lewis S, Louis C, Barillas-Mury C, Nusskern D, Rubin GM, Salzberg SL, Sutton GG, Topalis P, Wides R, Wincker P, Yandell M, Collins FH, Ribeiro J, Gelbart WM, Kafatos FC, Bork P, 2002. Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster. Science 298 : 149–159.
  42. Thomasova D, Ton LQ, Copley RR, Zdobnov EM, Wang X, Hong YS, Sim C, Bork P, Kafatos FC, Collins FH, 2002. Comparative genomic analysis in the region of a major Plasmodium-refractoriness locus of Anopheles gambiae. Proc Natl Acad Sci USA 99 : 8179–8184.
  43. Sharakhov IV, Serazin AC, Grushko OG, Dana A, Lobo N, Hillenmeyer ME, Westerman R, Romero-Severson J, Costantini C, Sagnon N, Collins FH, Besansky NJ, 2002. Inversions and gene order shuffling in Anopheles gambiae and A. funestus. Science 298 : 182–185.
  44. Ross HH, 1951. Conflict with Culex. Mosq News 11 : 128–132.
  45. Besansky NJ, Fahey GT, 1997. Utility of the white gene in estimating phylogenetic relationships among mosquitoes (Diptera:Culicidae). Mol Biol Evol 14 : 443–454.
  46. Myers EW, Sutton GG, Delcher AL, Dew IM, Fasulo DP, Flanigan MJ, Kravitz SA, Mobarry CM, Reinert KH, Remington KA, Anson EL, Bolanos RA, Chou HH, Jordan CM, Halpern AL, Lonardi S, Beasley EM, Brandon RC, Chen L, Dunn PJ, Lai Z, Liang Y, Nusskern DR, Zhan M, Zhang Q, Zheng X, Rubin GM, Adams MD, Venter JC, 2000. A whole-genome assembly of Drosophila. Science 287 : 2196–2204.
  47. Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, Nusskern DR, Wincker P, Clark AG, Ribeiro JM, Wides R, Salzberg SL, Loftus B, Yandell M, Majoros WH, Rusch DB, Lai Z, Kraft CL, Abril JF, Anthouard V, Arensburger P, Atkinson PW, Baden H, de Berardinis V, Baldwin D, Benes V, Biedler J, Blass C, Bolanos R, Boscus D, Barnstead M, Cai S, Center A, Chatuverdi K, Christophides GK, Chrystal MA, Clamp M, Cravchik A, Curwen V, Dana A, Delcher A, Dew I, Evans CA, Flanigan M, Grundschober-Freimoser A, Friedli L, Gu Z, Guan P, Guigo R, Hillenmeyer ME, Hladun SL, Hogan JR, Hong YS, Hoover J, Jaillon O, Ke Z, Kodira C, Kokoza E, Koutsos A, Letunic I, Levitsky A, Liang Y, Lin JJ, Lobo NF, Lopez JR, Malek JA, McIntosh TC, Meister S, Miller J, Mobarry C, Mongin E, Murphy SD, O’Brochta DA, Pfannkoch C, Qi R, Regier MA, Remington K, Shao H, Sharakhova MV, Sitter CD, Shetty J, Smith TJ, Strong R, Sun J, Thomasova D, Ton LQ, Topalis P, Tu Z, Unger MF, Walenz B, Wang A, Wang J, Wang M, Wang X, Woodford KJ, Wortman JR, Wu M, Yao A, Zdobnov EM, Zhang H, Zhao Q, Zhao S, Zhu SC, Zhimulev I, Coluzzi M, della Torre A, Roth CW, Louis C, Kalush F, Mural RJ, Myers EW, Adams MD, Smith HO, Broder S, Gardner MJ, Fraser CM, Birney E, Bork P, Brey PT, Venter JC, Weissenbach J, Kafatos FC, Collins FH, Hoffman SL, 2002. The genome sequence of the malaria mosquito Anopheles gambiae. Science 298 : 129–149.
  48. Kaufman TC, Severson DW, Robinson GE. 2002. The Anopheles genome and comparative insect genomics. Science 298 : 97–98.

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  • Received : 27 Dec 2002
  • Accepted : 03 Apr 2003

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