1921
Volume 69, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The development of comparative genetic maps in multiple species of mosquitoes could prove extremely useful in the search for those genes that contribute to mosquito vector competence or genes associated with other phenotypes of interest. To effectively compare these gene maps, markers must be developed that are based on chromosomal regions conserved throughout the Culicidae. We designed 35 polymerase chain reaction (PCR) primer pairs based upon orthlogous exons in and or . Twenty-three of the primers yielded a single PCR product in at least one dipteran, in addition to , when screened with genomic DNA from seven dipterans, including five mosquito species. Eight of the primers amplified a single PCR product in only , while four primer pairs gave no PCR product in any species. The 23 successful comparative anchor-tagged sequence primer pairs give broad genome coverage in , and more importantly demonstrate an efficient strategy for developing comparative anchor marker loci for any species of Culicidae.

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2003-07-01
2017-09-26
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  • Received : 27 Dec 2002
  • Accepted : 03 Apr 2003

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