Volume 69, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Eighty-nine ticks were collected from the islands of St. Kitts and Nevis in the Caribbean and preserved in 70% ethanol or local rum. After being washed in sterile water, their DNA was extracted and analyzed by a polymerase chain reaction (PCR) for DNA of spotted fever group rickettsiae and ehrlichiae. None of the tested ticks was positive in a PCR assay using the primers 16S EHRD and 16S EHRR for the 16S rRNA gene of spp.. Forty-one percent of the (36 of /89 of which 34 [47%] of 72 were adult males, 2 (13%) of 16 were adult females, and 0 (0%) of 1 were nymphs) were positive in a PCR assay using the primer pair 190-70 and 190-701 for the outer membrane protein A () gene of spotted fever group rickettsiae. All PCR amplification products obtained had 100% sequence homology with , the agent of African tick-bite fever.


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  • Received : 11 Dec 2002
  • Accepted : 26 Feb 2003

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