1921
Volume 69, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Molecular data on are very scarce, despite the fact that this genus includes all the species involved in the transmission of leishmaniasis in America. We examine the genetic relationships among eight morphologic groups within the genus and two species, using nine enzyme loci and the last 285 basepairs of the mitochondrial cytochrome b gene. The structure of the genetic variation among the species analyzed indicated a closer genetic relationship among members of a morphologic group than between members of different groups. The lower levels of variation recorded among these groups compared with that between and suggest a subgeneric status for all of these groups, including . A maximum likelihood tree for the allozyme data and a neighbor-joining consensus tree for the mitochondrial DNA sequences showed a general agreement with morphologic groups, with only minor differences. , and formed separate monophyletic groups. could not be separated from and both species, and grouped in different clades according to the host species they are found on.

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2003-11-01
2017-11-21
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References

  1. Young DG, Duncan MA, 1994. Guide to the Identification and Geographic Distribution of Lutzomyia Sand Flies in Mexico, the West Indies, Central and South America (Diptera: Psychodidae). Volume 2. Gainesville, FL: Associate Publishers.
  2. Lewis DJ, Young DG, Fairchild GB, Minter DM, 1977. Proposals for a stable classification of the Phlebotominae sandflies (Diptera:Psychodidae). Syst Entomol 2: 319–332.
  3. Ready PD, Day JC, de Souza AA, Rangel EF, Davies CR, 1997. Mitochondrial DNA characterization of populations of Lutzomyia whitmani (Diptera:Psychodidae) incriminated in the peri-domestic and silvatic transmission of Leishmania species in Brazil. Bull Entomol Res 87: 187–195.
  4. Theodor O, 1965. On the classification of American phlebotominae. J Med Entomol 2: 171–197.
  5. Ready PD, Fraiha H, Lainson R, Shaw JJ, 1980. Psychodopygus as a genus: reasons for a flexible classification of the phlebotomine sand flies (Diptera:Psychodidae). J Med Entomol 17: 75–88.
  6. Esseghir S, Ready PD, Killick-Kendrick R, Ben-Ismall R, 1997. Mitocondrial haplotypes and phylogeography of Phlebotomus vectors of Leishmania major. Insect Mol Biol 6 : 211–225.
  7. Dujardin JP, Le Pont F, Martinez E, 1999. Quantitative phenetics and taxonomy of some phlebotomine taxa. Mem Inst Oswaldo Cruz 94: 735–741.
  8. Dujardin JP, Le Pont F, Cruz M, Leon R, Tarrieu F, Guderian R, Echeverria R, Tibayrenc M, 1996. Criptic speciation in Lutzomya (Nyssomyia) trapidoi (Fairchild & Hertig) (Diptera: Psychodidae) detected by multilocus enzyme electrophoresis. Am J Trop Med Hyg 54: 42–45.
  9. Lins RMMA, Oliveira SG, Souza NA, de Queiroz RG, Justiniano SCB, Ward RD, Kyriacou CP, Peixoto AA, 2002. Molecular evolution of the cacophony IVS6 region in sandflies. Insect Mol Biol 11: 117–122.
  10. Mazzoni CJ, Gomes CA, Souza NA, de Queiroz RG, Justiniano SCB, Ward RD, Kyriacou CP, Peixoto AA, 2002. Molecular evolution of the period gene in sandflies. J Mol Evol 55: 553–562.
  11. Testa JM, Montoya-Lerma J, Cadena H, Oviedo M, Ready PD, 2002. Molecular identification of vectors of Leishmania in Colombia: Mitochondrial introgression in the Lutzomyia townsendi series. Acta Trop. 84: 205–218.
  12. Tesh RB, Guzman H, 1996. Sand flies and the agents they transmit. Beatty BJ, Marquadt WC, eds. The Biology of Disease Vectors. Niwot, CO: University Press of Colorado, 117–127.
  13. Torgerson D, Lampo M, Woo PTK, 2000. Cellulose acetate vs. polyacrylamide enzyme electrophoresis for the identification of 13 species of phlebotomine sand flies (Diptera:Psychodidae) from Venezuela. J Med Entomol 38: 501–509.
  14. Swofford DL, Selander RB, 1981. BIOSYS-1: a FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics. J Hered 72: 281–283.
  15. Sokal RS, Rohlf FJ, 1998. Biometry. The Principles and Practice of Statistics in Biological Research. New York: W. H. Freeman and Company.
  16. Felsenstein J, 1993. PHYLIP (Phylogeny Inference Package) version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle, WA.
  17. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG, 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24: 4876–4883.
  18. Excoffier L, Smouse P, Quattro J, 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131: 479–491.
  19. Schneider S, Roessli D, Excoffier L, 2000. Arlequin version 2.000. A software for population genetics data analysis. Available at http://anthropologie.unige.ch/arlequin/.
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  • Received : 13 Feb 2003
  • Accepted : 18 Jun 2003

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