1921
Volume 69, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Serodiagnosis of plague is very useful for its retrospective confirmation and for epidemiologic studies in humans and in rodents, since rats constitute the main natural reservoir of . We have developed a rapid test for the detection of IgG antibodies to fraction 1 (F1) based on immunochromatography and protein A to detect both human and rat IgG. When tested with reference human sera (35 positive and 37 negative), this assay showed a sensitivity of 94.3% and a specificity of 89.2%. When and reference sera (22 positive and 24 negative) were used, the sensitivity was 100% and the specificity was 91.7%. This simple serodiagnostic tool is of great potential value in the surveillance of plague. As far as we know, this test is the first of its kind designed for diagnosis of both humans and animals.

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2003-10-01
2017-09-20
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References

  1. Perry RD, Fetherson JD, 1997. Yersinia pestis, etiologic agent of plague. Clin Microbiol Rev 10 : 35–66.
  2. Chen TH, Meyer KF, 1966. An evaluation of Pasteurella pestis fraction 1 specific antibody for the confirmation of plague infections. Bull World Health Organ 34 : 911–918.
  3. Rasoamanana B, Leroy F, Boisier P, Rasolomaharo M, Buchy P, Carniel E, Chanteau S, 1997. Field evaluation of an IgG anti-F1 ELISA test for the serodiagnosis of human plague in Madagascar. Clin Diagn Lab Immunol 4 : 587–591.
  4. World Health Organization, 2000. Outbreak news. Wkly Epidemiol Rec 75 : 337–344.
  5. Boisier P, Rahalison L, Rasolomaharo M, Ratsitorahina M, Mahafaly M, Razafymahefa M, Duplantier JM, Ratsifasoamanana L, Chanteau S, 2002. Four successive annual outbreaks of bubonic plague in the coastal city of Mahajanga (Madagascar): epidemiological features. Emerg Infect Dis 8 : 311–316.
  6. Brubaker RR, 1970. Interconversion of purine mononucleotides in Pasteurella pestis. Infect Immun 11 : 446–454.
  7. Chanteau S, Rahalison L, Ratsitorahina M, Mahafaly M, Rasolomaharo M, Boisier P, O’Brien T, Aldrich J, Keheler A, Morgan C, Burans J, 2000. Early diagnosis of bubonic plague using F1 antigen capture ELISA assay and rapid immunogold disptick. Int J Med Microbiol 290 : 279–283.
  8. Chanteau S, Rahalison L, Ralafiarisoa L, Foulon J, Ratsitorahina M, Ratsifasoamanana L, Carniel E, Nato F, 2003. Development and testing of a rapid diagnostic test for bubonic and pneumonic plague. Lancet 361 : 211–216.
  9. Ratsitorahina M, Rabarijaona L, Chanteau S, Boisier P, 2000. Seroepidemiology of human plague in the Madagascar highlands. Trop Med Int Health 5 : 94–98.
  10. Shepherd A, Hummitzsch D, Leman A, Swanepoel R, Searle L, 1986. Comparative tests for detection of plague antigen and antibody in experimentally infected wild rodents. J Clin Microbiol 24 : 1075–1078.
  11. Harlow E, Lane D, 1991. Anticorps, un Manuel de Laboratoire. Paris: Pradel Éditions.
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  • Received : 27 Jan 2003
  • Accepted : 01 Jul 2003

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