Live-attenuated dengue vaccine development
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


As part of a larger vaccine study, peripheral blood mononuclear cells (PBMC) were collected from volunteers for analysis of vaccine- induced T cell responses. The PBMC were re-stimulated with live dengue virus and assayed for T1 or T2 memory cell responses. Re-stimulated PBMC from the volunteers predominantly secreted interferon-γ. Little interleukin-4 (IL-4) or IL-10 secretion was detected, indicating a T1 type of T cell response. The interferon-γ response was primarily serotype-specific with some serotype cross-reactivity. T cell depletion studies showed that the interferon-γ was being secreted by CD4+ T lymphocytes and/or by cells other than CD8+ T lymphocytes that were being stimulated by the CD4+ T lymphocytes. CD3+ or CD8+ T cell depletion showed that granzyme B mRNA expression correlated with the presence of CD4+ T lymphocytes. However, depletion of CD4+ T cells after four days of stimulation indicated that the granzyme B mRNA was produced by cells in culture other than lymphocytes. In summary, an antigen-specific T1 type T cell response was seen as a response to vaccination using live attenuated dengue virus.


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